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Q0ATN3

- BIOB_MARMM

UniProt

Q0ATN3 - BIOB_MARMM

Protein

Biotin synthase

Gene

bioB

Organism
Maricaulis maris (strain MCS10)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi270 – 2701Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMMAR394221:GHNB-60-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Mmar10_0058
    OrganismiMaricaulis maris (strain MCS10)
    Taxonomic identifieri394221 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeMaricaulis
    ProteomesiUP000001964: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 334334Biotin synthasePRO_0000381455Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi394221.Mmar10_0058.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0ATN3.
    SMRiQ0ATN3. Positions 14-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0ATN3-1 [UniParc]FASTAAdd to Basket

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    MSLLNDIDQL SQVRTDWTRE TAGEIYRAPF NDLIFAAQSV HRQCHPANQV    50
    QTSQLLSIKT GGCAEDCGYC NQSAHFDTGL KASKLMPLDD VLDAAKKAKD 100
    GGATRFCMGA AWRELKTRDE DVICDMISGV KSMGMETCVT LGMLTDSQAN 150
    KLREAGLDYY NHNLDTAPED YGRVISTRTY QDRIDTLSRV RGAGIHVCTG 200
    GIVGMGEDEA ARIGLLTELA SMDPHPESVP INHLVAVPNT PLGDSKPLDG 250
    IEFVRTIATA RILMPRSMVR LSAGREGMSR ELQALCFLAG ANSIFVGEEL 300
    LTTPNPEQNE DFDLFKSLGI EPMRLGETGT VPAE 334
    Length:334
    Mass (Da):36,385
    Last modified:October 17, 2006 - v1
    Checksum:i103BCB6E03DE5B53
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000449 Genomic DNA. Translation: ABI64354.1.
    RefSeqiYP_755292.1. NC_008347.1.

    Genome annotation databases

    EnsemblBacteriaiABI64354; ABI64354; Mmar10_0058.
    GeneIDi4283870.
    KEGGimmr:Mmar10_0058.
    PATRICi22448092. VBIMarMar77530_0060.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000449 Genomic DNA. Translation: ABI64354.1 .
    RefSeqi YP_755292.1. NC_008347.1.

    3D structure databases

    ProteinModelPortali Q0ATN3.
    SMRi Q0ATN3. Positions 14-324.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394221.Mmar10_0058.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI64354 ; ABI64354 ; Mmar10_0058 .
    GeneIDi 4283870.
    KEGGi mmr:Mmar10_0058.
    PATRICi 22448092. VBIMarMar77530_0060.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci MMAR394221:GHNB-60-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MCS10.

    Entry informationi

    Entry nameiBIOB_MARMM
    AccessioniPrimary (citable) accession number: Q0ATN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3