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Protein
Submitted name:

Uroporphyrin-III C-methyltransferase

Gene

Mmar10_0347

Organism
Maricaulis maris (strain MCS10)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.SAAS annotation

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.SAAS annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.SAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.SAAS annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.SAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrin-III C-methyltransferase (Mmar10_0347)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.SAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrin-III C-methyltransferase (Mmar10_0347)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.SAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrin-III C-methyltransferase (Mmar10_0347)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.SAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrin-III C-methyltransferase (Mmar10_0347)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei247Proton acceptorUniRule annotation1
Active sitei269Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseSAAS annotation, MethyltransferaseUniRule annotationSAAS annotationImported, Multifunctional enzymeSAAS annotation, OxidoreductaseSAAS annotation, Transferase
Biological processCobalamin biosynthesisSAAS annotation, Porphyrin biosynthesisSAAS annotation
LigandNADSAAS annotation, S-adenosyl-L-methionineSAAS annotation

Enzyme and pathway databases

BioCyciMMAR394221:G1G79-361-MONOMER
UniPathwayiUPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Submitted name:
Uroporphyrin-III C-methyltransferaseImported
Gene namesi
Ordered Locus Names:Mmar10_0347Imported
OrganismiMaricaulis maris (strain MCS10)Imported
Taxonomic identifieri394221 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeMaricaulis
Proteomesi
  • UP000001964 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi394221.Mmar10_0347

Structurei

3D structure databases

ProteinModelPortaliQ0ASU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 206CysG_dimeriserInterPro annotationAdd BLAST57
Domaini217 – 427TP_methylaseInterPro annotationAdd BLAST211

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.SAAS annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.SAAS annotation

Phylogenomic databases

eggNOGiENOG4105CB8 Bacteria
COG0007 LUCA
COG1648 LUCA
HOGENOMiHOG000290518
KOiK02302
OMAiFCVRADD
OrthoDBiPOG091H0526

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q0ASU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFPASIPL DGQTVIVVGQ GPMAEPKARL FASSPARLLW FTGETHDPVA
60 70 80 90 100
SDIAQYAKII RRVPTRRDFR GATLIFLAGG EDRALAKLAR WGRSQGALVN
110 120 130 140 150
IVDSPAASDF QTPAIVDRDG IVVSIASGGA APVLSVDIRA AIEQILPARI
160 170 180 190 200
GDIADLARGL RGTVKSVLGK FDDRRAFWER ALRGKARDLA LSGDKAGARR
210 220 230 240 250
EMLRELNGET SARAGIVHLV GAGPGDPDLL TLKAARLLRE ADVIVHDRLV
260 270 280 290 300
SQGVMDLARR DALRIDVGKT KGHHPVPQEQ IGEILVREAR KGQRVVRIKG
310 320 330 340 350
GDPFVFGRGG EELDVVRAAG IKAEVTPGIT AAVACAASAG IPLTHRDHAQ
360 370 380 390 400
SVTFASGVVK QDGPDADYRA LSAPNSTTVF YMGVGAAPDI QAKMIEAGRD
410 420 430 440 450
PATPVALVEN GTLDTERRVF GTLGDLAALV EREAISGPTI MIVGEVAGAA
460
LTSARASAGQ EQFA
Length:464
Mass (Da):48,907
Last modified:October 17, 2006 - v1
Checksum:i5E0A46082AFB64EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000449 Genomic DNA Translation: ABI64640.1
RefSeqiWP_011642287.1, NC_008347.1

Genome annotation databases

EnsemblBacteriaiABI64640; ABI64640; Mmar10_0347
KEGGimmr:Mmar10_0347

Similar proteinsi

Entry informationi

Entry nameiQ0ASU7_MARMM
AccessioniPrimary (citable) accession number: Q0ASU7
Entry historyiIntegrated into UniProtKB/TrEMBL: October 17, 2006
Last sequence update: October 17, 2006
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health