Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

Uroporphyrin-III C-methyltransferase

Gene

Mmar10_0347

Organism
Maricaulis maris (strain MCS10)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.SAAS annotation

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.SAAS annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.SAAS annotation
S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.SAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.SAAS annotation

Pathwayi

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: InterPro
  3. sirohydrochlorin ferrochelatase activity Source: InterPro
  4. uroporphyrin-III C-methyltransferase activity Source: InterPro

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. siroheme biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LyaseSAAS annotation, MethyltransferaseUniRule annotationSAAS annotation, OxidoreductaseSAAS annotation, Transferase

Keywords - Biological processi

Cobalamin biosynthesisSAAS annotation, Porphyrin biosynthesisSAAS annotation

Keywords - Ligandi

NADSAAS annotation, S-adenosyl-L-methionineSAAS annotation

Enzyme and pathway databases

BioCyciMMAR394221:GHNB-353-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Submitted name:
Uroporphyrin-III C-methyltransferaseImported
Gene namesi
Ordered Locus Names:Mmar10_0347Imported
OrganismiMaricaulis maris (strain MCS10)Imported
Taxonomic identifieri394221 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeMaricaulis
ProteomesiUP000001964 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi394221.Mmar10_0347.

Family & Domainsi

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiMEDETEC.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0ASU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFPASIPL DGQTVIVVGQ GPMAEPKARL FASSPARLLW FTGETHDPVA
60 70 80 90 100
SDIAQYAKII RRVPTRRDFR GATLIFLAGG EDRALAKLAR WGRSQGALVN
110 120 130 140 150
IVDSPAASDF QTPAIVDRDG IVVSIASGGA APVLSVDIRA AIEQILPARI
160 170 180 190 200
GDIADLARGL RGTVKSVLGK FDDRRAFWER ALRGKARDLA LSGDKAGARR
210 220 230 240 250
EMLRELNGET SARAGIVHLV GAGPGDPDLL TLKAARLLRE ADVIVHDRLV
260 270 280 290 300
SQGVMDLARR DALRIDVGKT KGHHPVPQEQ IGEILVREAR KGQRVVRIKG
310 320 330 340 350
GDPFVFGRGG EELDVVRAAG IKAEVTPGIT AAVACAASAG IPLTHRDHAQ
360 370 380 390 400
SVTFASGVVK QDGPDADYRA LSAPNSTTVF YMGVGAAPDI QAKMIEAGRD
410 420 430 440 450
PATPVALVEN GTLDTERRVF GTLGDLAALV EREAISGPTI MIVGEVAGAA
460
LTSARASAGQ EQFA
Length:464
Mass (Da):48,907
Last modified:October 17, 2006 - v1
Checksum:i5E0A46082AFB64EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000449 Genomic DNA. Translation: ABI64640.1.
RefSeqiYP_755578.1. NC_008347.1.

Genome annotation databases

EnsemblBacteriaiABI64640; ABI64640; Mmar10_0347.
KEGGimmr:Mmar10_0347.
PATRICi22448674. VBIMarMar77530_0347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000449 Genomic DNA. Translation: ABI64640.1.
RefSeqiYP_755578.1. NC_008347.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394221.Mmar10_0347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI64640; ABI64640; Mmar10_0347.
KEGGimmr:Mmar10_0347.
PATRICi22448674. VBIMarMar77530_0347.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiMEDETEC.
OrthoDBiEOG6DRPFR.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.
BioCyciMMAR394221:GHNB-353-MONOMER.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MCS10Imported.

Entry informationi

Entry nameiQ0ASU7_MARMM
AccessioniPrimary (citable) accession number: Q0ASU7
Entry historyi
Integrated into UniProtKB/TrEMBL: October 17, 2006
Last sequence update: October 17, 2006
Last modified: April 29, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeSAAS annotation, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.