Reviewed, UniProtKB/Swiss-Prot Q0AR08 (SYE1_MARMM)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Mmar10_0986

Organism

Maricaulis maris (strain MCS10) [Complete proteome] [HAMAP]

Taxonomic identifier

394221 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Maricaulis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367703
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AR08-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 950A30EC7BEEBB0C	FASTA	445	48,719
	10 20 30 40 50 60 MTKVRFAPSP TGKLHVGNVR TAIFNYLFAR KAGGTFVLRI DDTDVERSTK AYEDGIRADL 70 80 90 100 110 120 TWLGISWDET FKQSDRFDRY EAAAETLKAK GLLYPCYETG EDLDRKRRLQ MANGRPPVYD 130 140 150 160 170 180 RAALDLTDAE KAGFEAEGRK PHWRFKLSGN PVQWDDMVRG HVSIETSSLS DPILIREDGS 190 200 210 220 230 240 FLYTLPSVLD DIESEITHII RGEDHTTNSG AQIEIFEALG GKAPIFGHQA LLVGADGGKL 250 260 270 280 290 300 SKRTGSLGIA DLRDVDGLEP MAIMSLLARI GTSDPVEAFA DVEGILEGFD LGKLSRSPAR 310 320 330 340 350 360 FDDEELKRVN AKLLHAMPYD VAQPKLAVLG ADKGEAVWEA VRPNLETLAD AKAWAVLIDG 370 380 390 400 410 420 PVTPVIEEPD YAAAAAQHLP AGELDGDSWS VWTNALKEAT GRKGKQLFMP LRLMLTGEAR 430 440 GPEMAVLLPL IGRDKVLKRL AGEAA

References

[1]

"Complete sequence of Maricaulis maris MCS10." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Viollier P., Stephens C., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000449 Genomic DNA. Translation: ABI65279.1.
RefSeq	YP_756217.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AR08.
Genome annotation databases
GeneID	4285284.
GenomeReviews	Gene locus Mmar10_0986 in contig CP000449_GR.
KEGG	mmr:Mmar10_0986.
NMPDR	fig|394221.5.peg.963.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AR08.
OMA	LRLDDTD.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_MARMM

Accession

Primary (citable) accession number: Q0AR08

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)