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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Maricaulis maris (strain MCS10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

Zn2+UniRule annotation, Mg2+UniRule annotation, Co2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411SubstrateUniRule annotation
Binding sitei142 – 1421SubstrateUniRule annotation
Metal bindingi177 – 1771Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi222 – 2221Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi277 – 2771Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei285 – 2851SubstrateUniRule annotation
Binding sitei294 – 2941SubstrateUniRule annotation
Binding sitei303 – 3031SubstrateUniRule annotation

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. cobalt ion binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro
  5. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

BioCyciMMAR394221:GHNB-1246-MONOMER.
UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:Mmar10_1222
OrganismiMaricaulis maris (strain MCS10)
Taxonomic identifieri394221 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeMaricaulis
ProteomesiUP000001964: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3403404-hydroxythreonine-4-phosphate dehydrogenasePRO_1000081867Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi394221.Mmar10_1222.

Structurei

3D structure databases

ProteinModelPortaliQ0AQC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221592.
KOiK00097.
OMAiPIHKEAV.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0AQC2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQLSPVAVS MGDPAGIGPE IILKAWQSWR RRNGVPPLIA LGDIDAFTAT
60 70 80 90 100
AQALGLPAPC PLPTPTREAA ETLDGLPVFD VGVKIAAPVR PGQPDTANAA
110 120 130 140 150
CTKAAIETGV RLALDGQVSA LVTAPIAKSV MYEAGFAFPG HTEFLAELCA
160 170 180 190 200
DHPVDGPKGP AMMLAGGGLR VVLVTIHEPL VRALSLITPQ RVEATARITD
210 220 230 240 250
AALRRDFGIA RPRLALAGLN PHAGEGGALG DEEIDILDPL AARLRADGID
260 270 280 290 300
ITDAQPPDTL FHAEARAGYD AAICLYHDQG LIPVKTLDFH GGVNITLGLP
310 320 330 340
IVRTSPDHGT AFNIAGQGVA RPDSLLAALE QAVKIAECRA
Length:340
Mass (Da):35,313
Last modified:October 17, 2006 - v1
Checksum:iA848338DB11C6121
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000449 Genomic DNA. Translation: ABI65515.1.
RefSeqiWP_011643162.1. NC_008347.1.
YP_756453.1. NC_008347.1.

Genome annotation databases

EnsemblBacteriaiABI65515; ABI65515; Mmar10_1222.
GeneIDi4286475.
KEGGimmr:Mmar10_1222.
PATRICi22450514. VBIMarMar77530_1250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000449 Genomic DNA. Translation: ABI65515.1.
RefSeqiWP_011643162.1. NC_008347.1.
YP_756453.1. NC_008347.1.

3D structure databases

ProteinModelPortaliQ0AQC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394221.Mmar10_1222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI65515; ABI65515; Mmar10_1222.
GeneIDi4286475.
KEGGimmr:Mmar10_1222.
PATRICi22450514. VBIMarMar77530_1250.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221592.
KOiK00097.
OMAiPIHKEAV.
OrthoDBiEOG6GN6ZC.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.
BioCyciMMAR394221:GHNB-1246-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MCS10.

Entry informationi

Entry nameiPDXA_MARMM
AccessioniPrimary (citable) accession number: Q0AQC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: February 4, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.