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Q0APQ6 (ISPDF_MARMM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

  1. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
    EC=2.7.7.60
    Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    MEP cytidylyltransferase
    Short name=MCT
  2. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    Short name=MECDP-synthase
    Short name=MECPS
    EC=4.6.1.12
Gene names
Name:ispDF
Ordered Locus Names:Mmar10_1439
OrganismMaricaulis maris (strain MCS10) [Complete proteome] [HAMAP]
Taxonomic identifier394221 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Maricaulis

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520

Cofactor

Divalent metal cations By similarity. HAMAP MF_01520

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Sequence similarities

In the N-terminal section; belongs to the IspD family.

In the C-terminal section; belongs to the IspF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Bifunctional enzyme IspD/IspF HAMAP MF_01520
PRO_0000296749

Regions

Region1 – 2262262-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
Region227 – 3831572-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding2331Divalent metal cation By similarity
Metal binding2351Divalent metal cation By similarity
Metal binding2671Divalent metal cation By similarity
Site151Transition state stabilizer By similarity
Site221Transition state stabilizer By similarity
Site1521Positions MEP for the nucleophilic attack By similarity
Site2061Positions MEP for the nucleophilic attack By similarity
Site2591Transition state stabilizer By similarity
Site3581Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0APQ6 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: F5F0A75DABC804D4

FASTA38340,316
        10         20         30         40         50         60 
MKIAAVIVAA GRGERAGGGV PKQYRRLGGF FVLTHTLLRL LHREVFDSII VVVNPADADH 

        70         80         90        100        110        120 
IAAVEAELDI KLDTVPGGAT RTASVRAGLE RARDLGLDAV MIHDAARPFL GDALIDRLVK 

       130        140        150        160        170        180 
ALADHPAVIP ALPVADALFR GGDARMGDPV SRDGLYAAQT PQAFHLAPLL KAYARLGDTA 

       190        200        210        220        230        240 
LPDDAAVIRA AGGEVALVPG EAENFKLTTR ADFERAERQI MSETITVTGQ GYDVHRLEPA 

       250        260        270        280        290        300 
DVMWLCGVEI RAGLGLIGHS DADAGLHALT DAVLGAAGAG DIGQHFPPSD PQWKGAASDA 

       310        320        330        340        350        360 
FLLHAIKLLK QVGGELVHAD ITLIAERPKI GPHRDAMRAR VAELTGLPEK RVNIAATTTE 

       370        380 
KLGFTGRGEG LAAQAIVTAR LTT

« Hide

References

[1]"Complete sequence of Maricaulis maris MCS10."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Viollier P., Stephens C., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MCS10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000449 Genomic DNA. Translation: ABI65731.1.
RefSeqYP_756669.1. NC_008347.1.

3D structure databases

ProteinModelPortalQ0APQ6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0APQ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4285678.
GenomeReviewsGene locus Mmar10_1439 in contig CP000449_GR.
KEGGmmr:Mmar10_1439.
NMPDRfig|394221.5.peg.1404.
PATRIC22450972. VBIMarMar77530_1476.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAIVLIHDA.
PhylomeDBQ0APQ6.

Enzyme and pathway databases

BioCycMMAR394221:MMAR10_1439-MONOMER.

Family and domain databases

HAMAPMF_01520. IspDF.
[Tree]
InterProIPR023423. IpsF_dom.
IPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KOK12506.
PfamPF01128. IspD. 1 hit.
PF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMSSF69765. YgbB_synth. 1 hit.
TIGRFAMsTIGR00453. IspD. 1 hit.
TIGR00151. IspF. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
PS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPDF_MARMM
AccessionPrimary (citable) accession number: Q0APQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 17, 2006
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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