Reviewed, UniProtKB/Swiss-Prot Q0AMR3 (CARA_MARMM)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Mmar10_2132

Organism

Maricaulis maris (strain MCS10) [Complete proteome] [HAMAP]

Taxonomic identifier

394221 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Maricaulis

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138866
Regions
	Domain	202 – 390	189	Glutamine amidotransferase type-1
	Region	1 – 198	198	CPSase HAMAP MF_01209
Sites
	Active site	279	1	Nucleophile By similarity
	Active site	363	1	By similarity
	Active site	365	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AMR3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 22C04BB8C4D4C25A	FASTA	390	40,256
	10 20 30 40 50 60 MTSTPTPTPT GALALADGSV FLGHGTGATG IALGEVCFNT AMTGHQEILA DPSYAGQVVC 70 80 90 100 110 120 FTFPHVGNVG ANGEDEEAAS PQARKAAVGM IARAKITPPA SWRADTTFEE WLCTRGIVAL 130 140 150 160 170 180 TGIDTRALTR KIREGGMQMC AIAHDAAGNI DIEALKAAAA GAPTMEGREL AADVARTEGG 190 200 210 220 230 240 DWTEGNWTLG EGYAVGPEDG PRVVVLDYGV KANILRLLTG AGARVAVLPG KASIDDIKAL 250 260 270 280 290 300 NPDGVVVSNG PGDPAETGKY ALPTIKAVLD ANIPTLGICL GHQMLALAIG AKTAKMPQGH 310 320 330 340 350 360 HGANHPVKNH ETGQVEIVSM NHGFAVDGTT LPANAAETHV SLFDGSNSGF KLTDKPVWAV 370 380 390 QHHPEASPGP QDSFGVFDRF VGELKGRVEA

References

[1]

"Complete sequence of Maricaulis maris MCS10." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Viollier P., Stephens C., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000449 Genomic DNA. Translation: ABI66424.1.
RefSeq	YP_757362.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AMR3.
Genome annotation databases
GeneID	4285619.
GenomeReviews	Gene locus Mmar10_2132 in contig CP000449_GR.
KEGG	mmr:Mmar10_2132.
NMPDR	fig|394221.5.peg.2093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AMR3.
OMA	LFDGSNC.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_MARMM

Accession

Primary (citable) accession number: Q0AMR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)