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Q0AKF5 (SYL_MARMM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mmar10_2957
OrganismMaricaulis maris (strain MCS10) [Complete proteome] [HAMAP]
Taxonomic identifier394221 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeMaricaulis

Protein attributes

Sequence length865 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 865865Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009368

Regions

Motif44 – 5411"HIGH" region HAMAP-Rule MF_00049
Motif625 – 6295"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0AKF5 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: BD003E6FD3A8A8E8

FASTA86595,991
        10         20         30         40         50         60 
MTRYNPREAE PKWQSAWDAC DAFKAIGPSA SNNKPKYYVL EMFPYPSGRI HVGHSRNYTM 

        70         80         90        100        110        120 
GDVVARYKRA RGFDVLHPMG WDAFGLPAEN AARDRGVHPG AWTHDNIDAM RGQLQRLGLA 

       130        140        150        160        170        180 
LDWSREIATC DPSYYRHQQA IFLRLMERGL LHRKTAKVNW DPVDQTVLAN EQVIDGRGWR 

       190        200        210        220        230        240 
SGALVVQREL DQWFFKITEY ADELTDALEE LDRWPDKVRT MQSNWIGRSR GAEVSFPLTD 

       250        260        270        280        290        300 
SGLAEQFGAT IDVFTTRPDT LFGASFLALA PDHPIVKALA ADDPEIDRFM HECARMGTTA 

       310        320        330        340        350        360 
EEIEKAPKRG VDLGITVRHP FDENWELPVW SANFVLSGYG TGAIFGSPAG DQRDLDFARK 

       370        380        390        400        410        420 
YDLAVQPVVL PPEASADTHV IEDEAYTGPG TSYNSRFLDG LSTADALERA ITELEARKLG 

       430        440        450        460        470        480 
KGATSYRLRD WLVSRQRYWG CPIPVIHCAD CGVVPVPEDQ LPVVLPEDVT FDHPSNPLER 

       490        500        510        520        530        540 
HPTWKNADCP KCGKAGRRET DTLDTFVCSS WYFLRFTSPW SEDTPFLASD AEHWMPVDQY 

       550        560        570        580        590        600 
VGGIEHAILH LLYARFFTRA LNDAGLMNMK SGEPFAGLFT QGMVTHETYK SADGKWLSPE 

       610        620        630        640        650        660 
EVDLRKDGAV EMSTGKPVSV GAIEKMSKSK KNVVDLDAFI ESYGADAVRW FVLSDSPPER 

       670        680        690        700        710        720 
DVEYTDSGVE GVWRFVQRLW STVTSLPEGA PGPLTVAADA SGPALEIRRA AHKALHAVTD 

       730        740        750        760        770        780 
AIEGFRFNSA VAQVHDLVNV LRKYVPSDDA GIAAKTEALG ILARIIAPFV PHLAEECWEH 

       790        800        810        820        830        840 
LGGEGLVIDA AWPEADPALL VEDSVTLPVQ VNGKRRTEVT APKGAAQDVV QEMVMADQTV 

       850        860 
QRALEGLTVR KVIVVPDRIV NIVAG 

« Hide

References

[1]"Complete sequence of Maricaulis maris MCS10."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Viollier P., Stephens C., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MCS10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000449 Genomic DNA. Translation: ABI67238.1.
RefSeqYP_758176.1. NC_008347.1.

3D structure databases

ProteinModelPortalQ0AKF5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394221.Mmar10_2957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI67238; ABI67238; Mmar10_2957.
GeneID4286264.
KEGGmmr:Mmar10_2957.
PATRIC22454144. VBIMarMar77530_3037.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAREKNDWE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMMAR394221:GHNB-3011-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MARMM
AccessionPrimary (citable) accession number: Q0AKF5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries