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Q0AJ68

- TRMB_NITEC

UniProt

Q0AJ68 - TRMB_NITEC

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Nitrosomonas eutropha (strain C91)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561S-adenosyl-L-methionineUniRule annotation
Binding sitei81 – 811S-adenosyl-L-methionineUniRule annotation
Binding sitei108 – 1081S-adenosyl-L-methionineUniRule annotation
Active sitei131 – 1311By similarity
Binding sitei131 – 1311S-adenosyl-L-methionineUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNEUT335283:GHT6-331-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Neut_0322
OrganismiNitrosomonas eutropha (strain C91)
Taxonomic identifieri335283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000001966: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224tRNA (guanine-N(7)-)-methyltransferasePRO_0000288190Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi335283.Neut_0322.

Structurei

3D structure databases

ProteinModelPortaliQ0AJ68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 2054Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiLHIATDH.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0AJ68-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSHPPIRSY VLRQGYFSHA QRHAYESLLP RYGIPFSENP IDLDSIFGRT
60 70 80 90 100
APKILEIGSG MGETTVEIAA QHPEKDFVAI EVHAPGIGSL LDRIEKHGLT
110 120 130 140 150
NLRIIPHDAK LVLQHMLTGE SLDGIHIFFP DPWPKARHHK RRLIQSDFVS
160 170 180 190 200
LLCNRLKPGG YLHIATDWED YAIHSLHVLS SEQRLINTAA DYAPRPAYRP
210 220
LTKFEQRGMK LGHTIRDLIF TKAA
Length:224
Mass (Da):25,378
Last modified:October 17, 2006 - v1
Checksum:i16D1BB4ED2B512CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI58603.1.
RefSeqiYP_746568.1. NC_008344.1.

Genome annotation databases

EnsemblBacteriaiABI58603; ABI58603; Neut_0322.
GeneIDi4273827.
KEGGinet:Neut_0322.
PATRICi22717604. VBINitEut7577_0479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI58603.1 .
RefSeqi YP_746568.1. NC_008344.1.

3D structure databases

ProteinModelPortali Q0AJ68.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 335283.Neut_0322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI58603 ; ABI58603 ; Neut_0322 .
GeneIDi 4273827.
KEGGi net:Neut_0322.
PATRICi 22717604. VBINitEut7577_0479.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi LHIATDH.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci NEUT335283:GHT6-331-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation."
    Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.
    Environ. Microbiol. 9:2993-3007(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C91.

Entry informationi

Entry nameiTRMB_NITEC
AccessioniPrimary (citable) accession number: Q0AJ68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3