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Q0AIP0

- DAPF_NITEC

UniProt

Q0AIP0 - DAPF_NITEC

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Nitrosomonas eutropha (strain C91)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei49 – 491SubstrateUniRule annotation
    Binding sitei68 – 681SubstrateUniRule annotation
    Active sitei77 – 771Proton donor/acceptorUniRule annotation
    Binding sitei161 – 1611SubstrateUniRule annotation
    Sitei163 – 1631Important for catalytic activityUniRule annotation
    Binding sitei194 – 1941SubstrateUniRule annotation
    Sitei212 – 2121Important for catalytic activityUniRule annotation
    Active sitei221 – 2211Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciNEUT335283:GHT6-517-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Neut_0505
    OrganismiNitrosomonas eutropha (strain C91)
    Taxonomic identifieri335283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
    ProteomesiUP000001966: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278Diaminopimelate epimerasePRO_1000011915Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi77 ↔ 221

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi335283.Neut_0505.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0AIP0.
    SMRiQ0AIP0. Positions 3-278.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 112Substrate bindingUniRule annotation
    Regioni77 – 793Substrate bindingUniRule annotation
    Regioni212 – 2132Substrate bindingUniRule annotation
    Regioni222 – 2232Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKMRIFNN.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0AIP0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLQFTKMHG LGNDFIVINA INQPASLTFL DPATIRRLAD RHFGIGFDQL    50
    LIVEQAREGG DFRYRIFNAD GGEVEQCGNG ARCFARFVRD YNLTDKNTIR 100
    VETARGIITP TIENNGEVSV NMGVPQFEPA EIPFQAAQRM PVYPLQIGDK 150
    TIEISAVSIG NPHAVQIIPD IDLAPVTTEG PKIEAHPLFP ERVNAGFMQI 200
    IDRAHIRLRV FERGTGETLA CGTGACAAVV CGILRGLLDT TVQVAMHGGN 250
    LQIRWDGKDK PVWMTGPAIT VFEGTIDL 278
    Length:278
    Mass (Da):30,467
    Last modified:October 17, 2006 - v1
    Checksum:i39078C56CE6D780A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000450 Genomic DNA. Translation: ABI58781.1.
    RefSeqiYP_746746.1. NC_008344.1.

    Genome annotation databases

    EnsemblBacteriaiABI58781; ABI58781; Neut_0505.
    GeneIDi4272006.
    KEGGinet:Neut_0505.
    PATRICi22718027. VBINitEut7577_0687.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000450 Genomic DNA. Translation: ABI58781.1 .
    RefSeqi YP_746746.1. NC_008344.1.

    3D structure databases

    ProteinModelPortali Q0AIP0.
    SMRi Q0AIP0. Positions 3-278.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 335283.Neut_0505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI58781 ; ABI58781 ; Neut_0505 .
    GeneIDi 4272006.
    KEGGi net:Neut_0505.
    PATRICi 22718027. VBINitEut7577_0687.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KMRIFNN.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci NEUT335283:GHT6-517-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation."
      Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.
      Environ. Microbiol. 9:2993-3007(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C91.

    Entry informationi

    Entry nameiDAPF_NITEC
    AccessioniPrimary (citable) accession number: Q0AIP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3