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Protein

Glutamate 5-kinase

Gene

proB

Organism
Nitrosomonas eutropha (strain C91)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei154Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 175ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Neut_0958
OrganismiNitrosomonas eutropha (strain C91)
Taxonomic identifieri335283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi
  • UP000001966 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000810811 – 373Glutamate 5-kinaseAdd BLAST373

Proteomic databases

PRIDEiQ0AHG4

Interactioni

Protein-protein interaction databases

STRINGi335283.Neut_0958

Structurei

3D structure databases

ProteinModelPortaliQ0AHG4
SMRiQ0AHG4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 359PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q0AHG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSILQSAR RIIVKVGSSL VTNEGSGLDR QVLGGWAGQI SRLKKMGKEV
60 70 80 90 100
VLVSSGAVAE GMQRLGWKTR PAALYELQAA AAVGQMDLAQ AYADCFSEYK
110 120 130 140 150
LLTAQILLTH DDLSSRKRYL NARSTILTLL NLDIVPIINE NDTVVSDEIR
160 170 180 190 200
FGDNDNLAAL VANLIEAEVL VILTDQEGLF TGDPRKHADA TLISEISVSD
210 220 230 240 250
PHIETMAGGT SSGIGRGGMQ SKVMAARRAA RSGAHTVIAS GRVENVLVRL
260 270 280 290 300
ANGEAIGTSL LADMPVKVAR KLWLADHLQV RGSVVLDDGA SRALLSGGKS
310 320 330 340 350
LLPIGVVEVR GNFERGEAIS CLNTSGREIA RGLANYSARE SHKIMRRPSS
360 370
QIEAVLGYVG EYELVHRDNL VIL
Length:373
Mass (Da):40,016
Last modified:October 17, 2006 - v1
Checksum:i7BBA6B28864D12F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA Translation: ABI59218.1
RefSeqiWP_011634042.1, NC_008344.1

Genome annotation databases

EnsemblBacteriaiABI59218; ABI59218; Neut_0958
KEGGinet:Neut_0958

Similar proteinsi

Entry informationi

Entry nameiPROB_NITEC
AccessioniPrimary (citable) accession number: Q0AHG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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