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Q0AHC1 (CYSG_NITEC) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Siroheme synthase

Including the following 3 domains:

  1. Uroporphyrinogen-III C-methyltransferase
    Short name=Urogen III methylase
    EC=2.1.1.107
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name=UROM
  2. Precorrin-2 dehydrogenase
    EC=1.3.1.76
  3. Sirohydrochlorin ferrochelatase
    EC=4.99.1.4
Gene names
Name:cysG
Ordered Locus Names:Neut_1002
OrganismNitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP]
Taxonomic identifier335283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646

Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP MF_01646

Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP MF_01646

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646

Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646

Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

Belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Siroheme synthase HAMAP MF_01646
PRO_0000330527

Regions

Region220 – 462243Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

Sequences

Sequence LengthMass (Da)Tools
Q0AHC1 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 7BC46D5072A7793A

FASTA47652,002
        10         20         30         40         50         60 
MDYFPVFLNI KQRNCLIVGG GSVAARKAKL LLRAGAHIHV VSPTINAEFT GLLQQFSSIS 

        70         80         90        100        110        120 
HNAETFRPDH LQGRVLVIAA THDRATNQAV SVAARKAGIP VNVVDNPDLC TFIMPSILDR 

       130        140        150        160        170        180 
SPIIVAVSSG GGSPVLARLL RARLEALIPA AYGRLATYAA QFRGQVRQHF SRQENRRFFW 

       190        200        210        220        230        240 
EKMLQGPFAE MVFAGKDQAA QDYLLETLNN SVDQPPIGEV YLVGAGPGDP DLLTFRAMRL 

       250        260        270        280        290        300 
MQQADVVIYD RLVAPAILDM VRQDADRIYA GKERNRHTLP QTSINNLLIK LAQEGKRVLR 

       310        320        330        340        350        360 
LKGGDPFIFG RGGEEIETLS QHQIPFQVVP GITAASGVAS YAGIPLTHRD YAHSCVFVTG 

       370        380        390        400        410        420 
HLKDNTVQLD WSALARPNQT IVVYMGLLGV SELCRQLIAH GLPKTTPAAI IQQGTTPNQC 

       430        440        450        460        470 
VLTGTLATLP TIIQENPLKP PTLIIVGEVV KLRQRLAWFN STSESLGNTP GYSKHP

« Hide

References

[1]"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation."
Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.
Environ. Microbiol. 9:2993-3007(2007) [PubMed: 17991028] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C91.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000450 Genomic DNA. Translation: ABI59261.1.
RefSeqYP_747226.1. NC_008344.1.

3D structure databases

ProteinModelPortalQ0AHC1.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0AHC1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4274154.
GenomeReviewsGene locus Neut_1002 in contig CP000450_GR.
KEGGnet:Neut_1002.
NMPDRfig|335283.3.peg.2152.
PATRIC22719203. VBINitEut7577_1268.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0007.
HOGENOMHBG730212.
OMAMCRRDAE.
PhylomeDBQ0AHC1.
ProtClustDBCLSK585370.

Enzyme and pathway databases

BioCycNEUT335283:NEUT_1002-MONOMER.

Family and domain databases

HAMAPMF_01646. Siroheme_synth.
[Tree]
InterProIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA_cysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit.
G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.8.210. Sirohaem_synthase_dimer_dom. 1 hit.
KOK02302.
PfamPF10414. CysG_dimeriser. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMSSF53790. Cor/por_Metransf. 1 hit.
TIGRFAMsTIGR01469. CobA_cysG_Cterm. 1 hit.
TIGR01470. CysG_Nterm. 1 hit.
PROSITEPS00839. SUMT_1. False negative.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSG_NITEC
AccessionPrimary (citable) accession number: Q0AHC1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 17, 2006
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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