Q0AHA4 (TYSY_NITEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Neut_1021

Organism

Nitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP]

Taxonomic identifier

335283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_1000000639
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AHA4 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A04C69631D3DBE73	FASTA	264	30,373
	10 20 30 40 50 60 MHPYLDLMRH VLQYGHKKTD RTGTGTLSIF GYQMRFDLQQ GFPLVTTKQC HVKSIIHELL 70 80 90 100 110 120 WFLRGDTNID YLKKNGISIW NEWADKSGEL GPVYGHQWRS WTVSGDKSID QIAQVIQQIK 130 140 150 160 170 180 ATPDSRRMII SAWNVGDLDK MALAPCHVLF QFYVVDGKLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YSLLTLMIAQ CCNLKPGEFI HTFGDAHLYL NHLEQARLQL TREPKSLPIM ELNPAIRNIL 250 260 DFRYEDFTLH NYDPHPPIKA PVAV

References

[1]

"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation." Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X. Environ. Microbiol. 9:2993-3007(2007) [PubMed: 17991028] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C91.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000450 Genomic DNA. Translation: ABI59278.1.
RefSeq	YP_747243.1. NC_008344.1.
3D structure databases
ProteinModelPortal	Q0AHA4.
SMR	Q0AHA4. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AHA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4273864.
GenomeReviews	Gene locus Neut_1021 in contig CP000450_GR.
KEGG	net:Neut_1021.
NMPDR	fig|335283.3.peg.257.
PATRIC	22719255. VBINitEut7577_1294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	Q0AHA4.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	NEUT335283:NEUT_1021-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_NITEC

Accession

Primary (citable) accession number: Q0AHA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program