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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Nitrosomonas eutropha (strain C91)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:Neut_1266
OrganismiNitrosomonas eutropha (strain C91)
Taxonomic identifieri335283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi
  • UP000001966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002129861 – 929Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST929

Interactioni

Protein-protein interaction databases

STRINGi335283.Neut_1266.

Structurei

3D structure databases

ProteinModelPortaliQ0AGL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 422Adenylyl removaseUniRule annotationAdd BLAST422
Regioni429 – 929Adenylyl transferaseUniRule annotationAdd BLAST501

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q0AGL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPISTSRA ASIVSSILPY SRYLKRVLEN ESELQQELLE QLHNPFTREE
60 70 80 90 100
MLGFLQDSTA HLIDEADLHR LLRQLRKRVI LRLAARDLAG LADLNEVMAT
110 120 130 140 150
MTALADVTIQ FSLEFLHAAM VQPGHFGKPK GEKTGTEQQL LIVAMGKLGG
160 170 180 190 200
GELNVSSDVD LVFIYPEDGE TDGSKSITNH EFFLRLGRKL IASLNDYTVD
210 220 230 240 250
GYVFRVDMRL RPYGENSPLA ISLPMLEDYF VTQGREWERH AWIKSRVITG
260 270 280 290 300
SSIAEAALME LIVRPFVFRK YLDFEAYEAM RSLHTQLRKE VDRRELHDNI
310 320 330 340 350
KLGPGGIREI EFITQVFQLI RGGRDADLCI RPTLGVLQRL KQKQPLPEQT
360 370 380 390 400
VEELIEAYYF LRKLEHRLQY LDDQQTQNLP QHSDDQILIA KSMGFTDYTG
410 420 430 440 450
FLKHLDLHRQ NVTRHFEQIF AVRRKAIKLG TFARIRPEQT NDNEIVKVFS
460 470 480 490 500
NQLKTLGYID PDKITARVQQ FYDGTTFRQL SHPNQERILE LMPTLMEVVT
510 520 530 540 550
RFPPVEITLE RMLRLLEKIG QYSAYLALLQ EYPQTLPRVA KLVSISQWAS
560 570 580 590 600
DYLGSHPILL DELLAPSGLH TLPDWPTLKT ELIHQLHHGN IPKAQIIEWQ
610 620 630 640 650
MDVLRHFQHA QVFRLLAVDL EGNLLLETLS DHLTALADLI LDNVLQLAWQ
660 670 680 690 700
GLKKKHRKSP AFAIIGYGKL GGKELGYVSD LDIVYLYQDN HPDAMEIYTK
710 720 730 740 750
LAQNINLWLT SHTSAGVLYE TDLRLRPNGT SGLLVNSIEA FSLYQHEQAW
760 770 780 790 800
VWEHQALTRA RFVIGDSEVG EKFEQMRKNI LCQPRDMENL KREILMMRTK
810 820 830 840 850
MLEAHPNSTP LFDVKHDHGG IIDVEFIVQY LVLGYAHRYP QLTSNIGNIA
860 870 880 890 900
LLKLAGELGL TSAEKANAAF IAYRELRRTQ HQLRLSGIPE ATGTALPKDV
910 920
SQKFARVTSD YLSSARRAVF QLWEDVFGT
Length:929
Mass (Da):106,389
Last modified:October 17, 2006 - v1
Checksum:i90084B4FF322989E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI59518.1.
RefSeqiWP_011634337.1. NC_008344.1.

Genome annotation databases

EnsemblBacteriaiABI59518; ABI59518; Neut_1266.
KEGGinet:Neut_1266.
PATRICi22719835. VBINitEut7577_1580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI59518.1.
RefSeqiWP_011634337.1. NC_008344.1.

3D structure databases

ProteinModelPortaliQ0AGL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335283.Neut_1266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI59518; ABI59518; Neut_1266.
KEGGinet:Neut_1266.
PATRICi22719835. VBINitEut7577_1580.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_NITEC
AccessioniPrimary (citable) accession number: Q0AGL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 17, 2006
Last modified: March 15, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.