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Q0AFT3 (Q0AFT3_NITEC) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172
Ordered Locus Names:Neut_1555
OrganismNitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP] EMBL ABI59799.1
Taxonomic identifier335283 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6541Zinc By similarity HAMAP MF_00172
Metal binding6561Zinc By similarity HAMAP MF_00172
Metal binding7391Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
Q0AFT3 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 351210D1F86ACD9D

FASTA76987,862
        10         20         30         40         50         60 
MMVFRSCREN IMKILTHNLG FPRIGAQREL KKALEAHWKG QSEAAQLLAT AREIRASNWL 

        70         80         90        100        110        120 
SQQKSGIDLI PVGDFSLYDH ILDMTALLGA IPHRFEHTGD QVPLDLYFAM ARGTVNQPAM 

       130        140        150        160        170        180 
EMTKWFNTNY HYIVPEFDHN TRFHLASDWL FQEIKEAKVL GINTKVVLIG PLTYLYLGKE 

       190        200        210        220        230        240 
ITPDFQRLDL LPSLLPVYRE ILQQIVALGV EWVQIDEPIL ALDLEQPWLD SFTEAYRALN 

       250        260        270        280        290        300 
DTGSRLLLTS YFGAVDHHLA LLENLPVDGL HIDLSSAPEQ LESFLTRNFS SKILSLGCID 

       310        320        330        340        350        360 
GRNIWRTDLS QKLENLAQTV SRFKGNLWIA PSCSLLHCPV DLSLETKLDP EIRNWLAFST 

       370        380        390        400        410        420 
QKLEEIATLG LGLYQGKESI QEILTASNQA NQSRKTSCRI HNPVVHHRIN HLTERDSQRT 

       430        440        450        460        470        480 
HPFAIRKQQQ QQRFNLPLLP TTTIGSFPQT SAIRQVRAAF KKNELSHLEY LSAMRQEICE 

       490        500        510        520        530        540 
TIRKQEEIGL DVLVHGEPER NDMVEYFGEQ LWGYAFTENG WVQSYGSRCV KPPILYGDVY 

       550        560        570        580        590        600 
RPEAMTVDWI SYAQSQTSRP VKGMLTGPVT MLFWSFVRDD QPRADTALQL ALAIRDEVTD 

       610        620        630        640        650        660 
LEKAGIGMIQ IDEPAFREGL PLRRQDWQHY LDWAVKAFRV ASSGVKDETQ IHTHMCYSEF 

       670        680        690        700        710        720 
HDILPAIAEL DADVITIETS RSRMELLDAF VKFSYPNEIG PGVYDIHSPR IPDTSEMLEL 

       730        740        750        760 
LKKASHHIDP KLLWVNPDCG LKTRNWPETR TALQKMVDCA KSLRSALQT

« Hide

References

[1]"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation."
Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.
Environ. Microbiol. 9:2993-3007(2007) [PubMed: 17991028] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000450 Genomic DNA. Translation: ABI59799.1.
RefSeqYP_747764.1. NC_008344.1.

3D structure databases

ProteinModelPortalQ0AFT3.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0AFT3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4272448.
GenomeReviewsGene locus Neut_1555 in contig CP000450_GR.
KEGGnet:Neut_1555.
NMPDRfig|335283.3.peg.1859.
PATRIC22720512. VBINitEut7577_1913.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0620.
HOGENOMHBG287495.
OMANIWANDF.
PhylomeDBQ0AFT3.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ0AFT3_NITEC
AccessionPrimary (citable) accession number: Q0AFT3
Entry history
Integrated into UniProtKB/TrEMBL: October 17, 2006
Last sequence update: October 17, 2006
Last modified: December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info