Q0AFN4 (SYR_NITEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Neut_1605

Organism

Nitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP]

Taxonomic identifier

335283 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas › Nitrosomonas eutropha

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_1000018076
Regions
	Motif	131 – 141	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q0AFN4 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: BA3B6F4517BEB060	FASTA	586	65,632
	10 20 30 40 50 60 MATTIPFDFR SHCVQLLAQA VKQVLPEETE IHIELQRPKQ MDHGDYSTNL AMKLAKRLRR 70 80 90 100 110 120 NPLELAKVLI GALPDSPYVG KTVVAGGGFV NFFLKKTAKQ QFLLTVLQAA DSFGHSKLGA 130 140 150 160 170 180 GKTIQIEFVS ANPTGPLHVG HGRGAAFGAS LANIMAAAGY AVTREFYVND AGRQMDILAL 190 200 210 220 230 240 STWLRYLDLC GIALPFPSNA YQGRYVVDMA LEIHRIHGDR YAHYSDSPTQ QLAEINAKAA 250 260 270 280 290 300 ADGEDEYLDN LITAAKSILG EDYAYLHNFV LTEQLNDCRN DLMEFGVEFE NWFSEQSLFD 310 320 330 340 350 360 SGMVARAVQL LDDKKLLYRQ DGALWFQSTG FGDEKDRVVQ RENGQYTYFA SDIAYHLSKY 370 380 390 400 410 420 ERGFDYMLNI WGADHHGYIS RVKGAIEALS LDSDKLEIAL VQFAVLYRDG NKISMSTRSG 430 440 450 460 470 480 EFVTLRQLRQ EVGNDAARFF YVLRKSDQHL DFDLDLAKSQ SNDNPVYYVQ YAHARICSVL 490 500 510 520 530 540 EQWGGAVDIF GRAETELLTN PAELVLLQKI IDFPDTIEAA AKEHAPHLIA FFLRELASEF 550 560 570 580 HSYYNSTRFL IPDEALKIAR LALISAVCQV LSKGLSLLGV TSPRKM

References

[1]

"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation." Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X. Environ. Microbiol. 9:2993-3007(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C91.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000450 Genomic DNA. Translation: ABI59848.1.
RefSeq	YP_747813.1. NC_008344.1.
3D structure databases
ProteinModelPortal	Q0AFN4.
ModBase	Search...
Protein-protein interaction databases
STRING	335283.Neut_1605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI59848; ABI59848; Neut_1605.
GeneID	4272093.
KEGG	net:Neut_1605.
PATRIC	22720628. VBINitEut7577_1969.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247214.
KO	K01887.
OMA	NPNGPLH.
OrthoDB	EOG6JB13C.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	NEUT335283:GHT6-1636-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF47323. SSF47323. 1 hit. SSF55190. SSF55190. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_NITEC

Accession

Primary (citable) accession number: Q0AFN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	November 13, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program