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Protein

Dihydroorotase

Gene

pyrC

Organism
Nitrosomonas eutropha (strain C91)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Zinc 1; via tele nitrogenUniRule annotation
Metal bindingi16 – 161Zinc 1; via tele nitrogenUniRule annotation
Metal bindingi102 – 1021Zinc 1; via carbamate groupUniRule annotation
Metal bindingi102 – 1021Zinc 2; via carbamate groupUniRule annotation
Metal bindingi139 – 1391Zinc 2; via pros nitrogenUniRule annotation
Metal bindingi177 – 1771Zinc 2; via tele nitrogenUniRule annotation
Metal bindingi250 – 2501Zinc 1UniRule annotation

GO - Molecular functioni

  1. dihydroorotase activity Source: UniProtKB-HAMAP
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. pyrimidine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciNEUT335283:GHT6-1692-MONOMER.
UniPathwayiUPA00070; UER00117.

Protein family/group databases

MEROPSiM38.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
DihydroorotaseUniRule annotation (EC:3.5.2.3UniRule annotation)
Short name:
DHOaseUniRule annotation
Gene namesi
Name:pyrCUniRule annotation
Ordered Locus Names:Neut_1659
OrganismiNitrosomonas eutropha (strain C91)
Taxonomic identifieri335283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000001966: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345DihydroorotasePRO_1000024025Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi335283.Neut_1659.

Structurei

3D structure databases

ProteinModelPortaliQ0AFI1.
SMRiQ0AFI1. Positions 2-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DHOase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0418.
HOGENOMiHOG000256259.
KOiK01465.
OMAiAFEQRNA.
OrthoDBiEOG6TFCMH.

Family and domain databases

HAMAPiMF_00219. PyrC_type1.
InterProiIPR006680. Amidohydro_1.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001237. DHOdimr. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0AFI1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNKLTFTRP DDWHLHLRDG KAMQSVLPDT ARRFARAMIM PNLKLPVVTT
60 70 80 90 100
EQAAAYRARI LSALPQELVG QFEPLMTLYL TDTTTPEEIS RAKASGIVQA
110 120 130 140 150
VKLYPAGATT HSDAGVTDID RCKATLAMME KLDMPLLVHG EVVDPAVDIF
160 170 180 190 200
DREKIFIDRV LIPLLQRFPG LRVVFEHITT REAVEFVQSI SNRIAATITA
210 220 230 240 250
HHLMLNRNAL FTGGLQPHHY CLPVLKREIH RQALVAAATS GHSRFFLGTD
260 270 280 290 300
SAPHPLKDKE SACGCAGIYS AHAAIEFYAE IFEQAGRLDR LEAFASFYGP
310 320 330 340
DFYGLPRNTD QISLSKESWQ IPGEVEFGGD RLVPLRAGEQ VCWRL
Length:345
Mass (Da):38,476
Last modified:October 17, 2006 - v1
Checksum:i0AD34C94361246F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI59901.1.
RefSeqiYP_747866.1. NC_008344.1.

Genome annotation databases

EnsemblBacteriaiABI59901; ABI59901; Neut_1659.
GeneIDi4273812.
KEGGinet:Neut_1659.
PATRICi22720754. VBINitEut7577_2030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI59901.1.
RefSeqiYP_747866.1. NC_008344.1.

3D structure databases

ProteinModelPortaliQ0AFI1.
SMRiQ0AFI1. Positions 2-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335283.Neut_1659.

Protein family/group databases

MEROPSiM38.A02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI59901; ABI59901; Neut_1659.
GeneIDi4273812.
KEGGinet:Neut_1659.
PATRICi22720754. VBINitEut7577_2030.

Phylogenomic databases

eggNOGiCOG0418.
HOGENOMiHOG000256259.
KOiK01465.
OMAiAFEQRNA.
OrthoDBiEOG6TFCMH.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00117.
BioCyciNEUT335283:GHT6-1692-MONOMER.

Family and domain databases

HAMAPiMF_00219. PyrC_type1.
InterProiIPR006680. Amidohydro_1.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001237. DHOdimr. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation."
    Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.
    Environ. Microbiol. 9:2993-3007(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C91.

Entry informationi

Entry nameiPYRC_NITEC
AccessioniPrimary (citable) accession number: Q0AFI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: January 7, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.