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Protein

Dihydroorotase

Gene

pyrC

Organism
Nitrosomonas eutropha (strain C91)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase (glutamine-hydrolyzing) (Neut_0455), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14Zinc 1; via tele nitrogenUniRule annotation1
Metal bindingi16Zinc 1; via tele nitrogenUniRule annotation1
Metal bindingi102Zinc 1; via carbamate groupUniRule annotation1
Metal bindingi102Zinc 2; via carbamate groupUniRule annotation1
Metal bindingi139Zinc 2; via pros nitrogenUniRule annotation1
Metal bindingi177Zinc 2; via tele nitrogenUniRule annotation1
Metal bindingi250Zinc 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00070; UER00117.

Names & Taxonomyi

Protein namesi
Recommended name:
DihydroorotaseUniRule annotation (EC:3.5.2.3UniRule annotation)
Short name:
DHOaseUniRule annotation
Gene namesi
Name:pyrCUniRule annotation
Ordered Locus Names:Neut_1659
OrganismiNitrosomonas eutropha (strain C91)
Taxonomic identifieri335283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi
  • UP000001966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000240251 – 345DihydroorotaseAdd BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102N6-carboxylysineUniRule annotation1

Proteomic databases

PRIDEiQ0AFI1.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi335283.Neut_1659.

Structurei

3D structure databases

ProteinModelPortaliQ0AFI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DHOase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EKE. Bacteria.
COG0418. LUCA.
HOGENOMiHOG000256259.
KOiK01465.
OMAiYAEAFEQ.
OrthoDBiPOG091H06EN.

Family and domain databases

CDDicd01294. DHOase. 1 hit.
HAMAPiMF_00219. PyrC_type1. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11647:SF59. PTHR11647:SF59. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001237. DHOdimr. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0AFI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNKLTFTRP DDWHLHLRDG KAMQSVLPDT ARRFARAMIM PNLKLPVVTT
60 70 80 90 100
EQAAAYRARI LSALPQELVG QFEPLMTLYL TDTTTPEEIS RAKASGIVQA
110 120 130 140 150
VKLYPAGATT HSDAGVTDID RCKATLAMME KLDMPLLVHG EVVDPAVDIF
160 170 180 190 200
DREKIFIDRV LIPLLQRFPG LRVVFEHITT REAVEFVQSI SNRIAATITA
210 220 230 240 250
HHLMLNRNAL FTGGLQPHHY CLPVLKREIH RQALVAAATS GHSRFFLGTD
260 270 280 290 300
SAPHPLKDKE SACGCAGIYS AHAAIEFYAE IFEQAGRLDR LEAFASFYGP
310 320 330 340
DFYGLPRNTD QISLSKESWQ IPGEVEFGGD RLVPLRAGEQ VCWRL
Length:345
Mass (Da):38,476
Last modified:October 17, 2006 - v1
Checksum:i0AD34C94361246F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI59901.1.
RefSeqiWP_011634707.1. NC_008344.1.

Genome annotation databases

EnsemblBacteriaiABI59901; ABI59901; Neut_1659.
KEGGinet:Neut_1659.
PATRICi22720754. VBINitEut7577_2030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000450 Genomic DNA. Translation: ABI59901.1.
RefSeqiWP_011634707.1. NC_008344.1.

3D structure databases

ProteinModelPortaliQ0AFI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335283.Neut_1659.

Proteomic databases

PRIDEiQ0AFI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI59901; ABI59901; Neut_1659.
KEGGinet:Neut_1659.
PATRICi22720754. VBINitEut7577_2030.

Phylogenomic databases

eggNOGiENOG4105EKE. Bacteria.
COG0418. LUCA.
HOGENOMiHOG000256259.
KOiK01465.
OMAiYAEAFEQ.
OrthoDBiPOG091H06EN.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00117.

Family and domain databases

CDDicd01294. DHOase. 1 hit.
HAMAPiMF_00219. PyrC_type1. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11647:SF59. PTHR11647:SF59. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001237. DHOdimr. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRC_NITEC
AccessioniPrimary (citable) accession number: Q0AFI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.