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Q0AF74 (NAGZ_NITEC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:Neut_1774
OrganismNitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP]
Taxonomic identifier335283 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000005658

Regions

Region168 – 1692Substrate binding By similarity

Sites

Active site1811Proton donor/acceptor By similarity
Active site2521Nucleophile By similarity
Binding site641Substrate By similarity
Binding site721Substrate By similarity
Binding site1381Substrate By similarity
Site1791Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0AF74 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: BFAB788AE8702C38

FASTA34838,310
        10         20         30         40         50         60 
MFLGPLMLDI AGTTLTETDR VQLSHPLVGG VILFARNYES PAQLSELTAS IHALRSPPLL 

        70         80         90        100        110        120 
IAVDQEGGRV QRFRDGFTRL PPMRTLGEIQ DRNPDLSLHL ARQIGYVLAA ELKACGVDVS 

       130        140        150        160        170        180 
FTPVLDLDCE QSSVIGDRAF YREPQVVAEL AHALMSGLQS VGMVAVGKHF PGHGAIQADT 

       190        200        210        220        230        240 
HVETAIDSRN YTDIEKKDLT PFRRMIDTGL SGIMAAHVIY PAIDPNPAGF SSKWLQDILR 

       250        260        270        280        290        300 
NELGFRGCIF SDDLCMQAAR NYGSITHRAE QALQAGCNMV LICNDPDSAD ELLTSLQWEF 

       310        320        330        340 
SAVDTARLEH MRGQQTVHSM AQLHEMERFI RATEEISRIS LANISVSV 

« Hide

References

[1]"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation."
Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.
Environ. Microbiol. 9:2993-3007(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C91.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000450 Genomic DNA. Translation: ABI60008.1.
RefSeqYP_747973.1. NC_008344.1.

3D structure databases

ProteinModelPortalQ0AF74.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335283.Neut_1774.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI60008; ABI60008; Neut_1774.
GeneID4273123.
KEGGnet:Neut_1774.
PATRIC22721030. VBINitEut7577_2165.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAIPDIDYD.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycNEUT335283:GHT6-1809-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNAGZ_NITEC
AccessionPrimary (citable) accession number: Q0AF74
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries