Q0AES9 (SYM_NITEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Neut_1921

Organism

Nitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP]

Taxonomic identifier

335283 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas › Nitrosomonas eutropha

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_0000331856
Regions
	Domain	600 – 701	102	tRNA-binding
	Motif	13 – 23	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	336 – 340	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	157	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AES9 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 6FA6809DC30A51CB	FASTA	701	79,411
	10 20 30 40 50 60 MTVRNILVTS ALPYANGSIH LGHLVEYIQT DIWVRFQKMQ GHTVYYVCAD DAHGTPIMLR 70 80 90 100 110 120 AEREGISPEA LIARVYTEHL RDFTGFHIAF DQYYSTHSDE TRYYAEDIYQ KLKAAGLIAI 130 140 150 160 170 180 RSIEQLYDPV KNLFLPDRFV KGECPKCGAV DQYGDSCEAC GAAYTPTELK NPYSAISGAT 190 200 210 220 230 240 PIRKASEHFF FKLSDPRCSD FLRKWTRSGN HLQVEAANKM AEWLGEEGEN KLSDWDISRD 250 260 270 280 290 300 APYFGFEIPG ETDKYFYVWL DAPIGYMGSF KKLCTEQGID FDQYWKKGAT TELYHFIGKD 310 320 330 340 350 360 ILYFHALFWP AMLENAGYRT PTQIFAHGFL TVNGEKMSKS RGTFITAESY LQQRLNPEWL 370 380 390 400 410 420 RYYYAAKLNG SMEDIDLNLE DFVTRVNADL VGKYINIASR CAGFISKCFD GKLVAGEDYQ 430 440 450 460 470 480 LLQQTVDEYF SSWQPGVIEA AYEARDFSAA IRHIMKRTDE VNELIHVLAP WEIAKDETRE 490 500 510 520 530 540 RELHRACSLG IQMFYLLSCY LKPVLPYTAE RIEHFLNWMA LGWPRQQAGQ PLSTMLLPAG 550 560 570 580 590 600 HTINTYQHLI TRIDPKQIAA LTEANRQTMT TSNDTHSLTR HAEAQQRVIA PIAETISIED 610 620 630 640 650 660 FSKIDLRIAR ILDAQHVPGA DKLLQLTLDI GSEQRSVFAG IKSAYDPEQL KGRLTVMVAN 670 680 690 700 LAPRKMKFGL SEGMVLAASG ESGGPFLLAP DSGAQPGMRV K

References

[1]

"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation." Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X. Environ. Microbiol. 9:2993-3007(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C91.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000450 Genomic DNA. Translation: ABI60153.1.
RefSeq	YP_748118.1. NC_008344.1.
3D structure databases
ProteinModelPortal	Q0AES9.
SMR	Q0AES9. Positions 4-567.
ModBase	Search...
Protein-protein interaction databases
STRING	335283.Neut_1921.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI60153; ABI60153; Neut_1921.
GeneID	4273343.
KEGG	net:Neut_1921.
PATRIC	22721388. VBINitEut7577_2340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200400.
KO	K01874.
OMA	HEIAPFK.
ProtClustDB	PRK00133.
Enzyme and pathway databases
BioCyc	NEUT335283:GHT6-1966-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_NITEC

Accession

Primary (citable) accession number: Q0AES9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program