Reviewed, UniProtKB/Swiss-Prot Q0ADU3 (TPMT_NITEC)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Neut_2272

Organism

Nitrosomonas eutropha (strain C91) [Complete proteome] [HAMAP]

Taxonomic identifier

335283 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047207
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0ADU3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 0E41C738E647D738	FASTA	220	24,704
	10 20 30 40 50 60 MEHEFWLQSW HEGRTGFHQL RVQPLLQKYW PTLDLPTGSK IFVPLTGKSL DMAWLAAQGY 70 80 90 100 110 120 RVLGVELSLL AVQQFFAEHG LKPAVRESHY GTHYTARNIE VICGDTFALD AALLSDCSGI 130 140 150 160 170 180 YDRAALIALP PELRVPYINE LMTCLPAGCS GLLITLEYQQ QEMVGPPFSV EEAEVLKCYS 190 200 210 220 PRWCVKLLER NDILPQEPGF AARGLTKLAT AVYQLQRLAV

References

[1]

"Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas eutropha C91: implications for niche adaptation." Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S., Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X. Environ. Microbiol. 9:2993-3007(2007) [PubMed: 17991028] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000450 Genomic DNA. Translation: ABI60489.1.
RefSeq	YP_748454.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4274036.
GenomeReviews	Gene locus Neut_2272 in contig CP000450_GR.
KEGG	net:Neut_2272.
NMPDR	fig|335283.3.peg.73.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0ADU3. PPFAVSP.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_NITEC

Accession

Primary (citable) accession number: Q0ADU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)