ID   TRMB_ALHEH              Reviewed;         237 AA.
AC   Q0ACP5;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
DE            EC=2.1.1.33;
DE   AltName: Full=tRNA(m7G46)-methyltransferase;
GN   Name=trmB; OrderedLocusNames=Mlg_0033;
OS   Alkalilimnicola ehrlichei (strain MLHE-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Alkalilimnicola.
OX   NCBI_TaxID=187272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T.,
RA   King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T.,
RA   Xie G., Stolz J.F., Richardson P.;
RT   "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at
CC       position 46 (m7G46) in tRNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(7)-methylguanine.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. TrmB
CC       family.
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DR   EMBL; CP000453; ABI55392.1; -; Genomic_DNA.
DR   RefSeq; YP_740882.1; -.
DR   GeneID; 4268890; -.
DR   GenomeReviews; CP000453_GR; Mlg_0033.
DR   KEGG; aeh:Mlg_0033; -.
DR   NMPDR; fig|187272.6.peg.32; -.
DR   HOGENOM; Q0ACP5; -.
DR   OMA; Q0ACP5; TKFENRG.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_01057; -; 1.
DR   InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase.
DR   PANTHER; PTHR23417:SF1; Methyltransf_4; 1.
DR   Pfam; PF02390; Methyltransf_4; 1.
DR   TIGRFAMs; TIGR00091; CHP91; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    237       tRNA (guanine-N(7)-)-methyltransferase.
FT                                /FTId=PRO_0000288116.
FT   REGION      212    215       Substrate binding (Potential).
FT   BINDING      65     65       S-adenosyl-L-methionine (By similarity).
FT   BINDING      90     90       S-adenosyl-L-methionine (By similarity).
FT   BINDING     117    117       S-adenosyl-L-methionine (By similarity).
FT   BINDING     140    140       S-adenosyl-L-methionine (By similarity).
FT   BINDING     144    144       Substrate (By similarity).
FT   BINDING     176    176       Substrate (Potential).
SQ   SEQUENCE   237 AA;  27016 MW;  00A03E61CD8CA2E2 CRC64;
     MERRADAATG KRPIRSFVRR EGRLTAGQQR ALELLWPAFG LERPPAGQPL DLDRAFGRRA
     PRILEIGFGN GESLAEQAAT HPERDYLGIE VHRPGVGHLL MEVEKRHLGN VRVMMADAAE
     VLAHHIPDGS LHGVQLFFPD PWPKKRHHKR RLVQPQWVRA VAAKLAPGGF LHLATDWADY
     AEHMLDVLEA EPDLENTCGP RQFSPRGERP ETKFERRGLR KGHQVFDLYY RKREHPG
//