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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651S-adenosyl-L-methionineUniRule annotation
Binding sitei90 – 901S-adenosyl-L-methionineUniRule annotation
Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation
Active sitei140 – 1401By similarity
Binding sitei140 – 1401S-adenosyl-L-methionineUniRule annotation
Binding sitei144 – 1441SubstrateUniRule annotation
Binding sitei176 – 1761SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAEHR187272:GHAX-33-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Mlg_0033
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
ProteomesiUP000001962 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237tRNA (guanine-N(7)-)-methyltransferasePRO_0000288116Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187272.Mlg_0033.

Structurei

3D structure databases

ProteinModelPortaliQ0ACP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2154Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0ACP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRADAATG KRPIRSFVRR EGRLTAGQQR ALELLWPAFG LERPPAGQPL
60 70 80 90 100
DLDRAFGRRA PRILEIGFGN GESLAEQAAT HPERDYLGIE VHRPGVGHLL
110 120 130 140 150
MEVEKRHLGN VRVMMADAAE VLAHHIPDGS LHGVQLFFPD PWPKKRHHKR
160 170 180 190 200
RLVQPQWVRA VAAKLAPGGF LHLATDWADY AEHMLDVLEA EPDLENTCGP
210 220 230
RQFSPRGERP ETKFERRGLR KGHQVFDLYY RKREHPG
Length:237
Mass (Da):27,016
Last modified:October 17, 2006 - v1
Checksum:i00A03E61CD8CA2E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI55392.1.
RefSeqiWP_011627788.1. NC_008340.1.

Genome annotation databases

EnsemblBacteriaiABI55392; ABI55392; Mlg_0033.
KEGGiaeh:Mlg_0033.
PATRICi20859236. VBIAlkEhr114327_0033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI55392.1.
RefSeqiWP_011627788.1. NC_008340.1.

3D structure databases

ProteinModelPortaliQ0ACP5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187272.Mlg_0033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI55392; ABI55392; Mlg_0033.
KEGGiaeh:Mlg_0033.
PATRICi20859236. VBIAlkEhr114327_0033.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciAEHR187272:GHAX-33-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1101 / DSM 17681 / MLHE-1.

Entry informationi

Entry nameiTRMB_ALKEH
AccessioniPrimary (citable) accession number: Q0ACP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 17, 2006
Last modified: July 22, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.