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Q0ACP5 (TRMB_ALKEH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Mlg_0033
OrganismAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) [Complete proteome] [HAMAP]
Taxonomic identifier187272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288116

Regions

Region212 – 2154Substrate binding By similarity

Sites

Active site1401 By similarity
Binding site651S-adenosyl-L-methionine By similarity
Binding site901S-adenosyl-L-methionine By similarity
Binding site1171S-adenosyl-L-methionine By similarity
Binding site1401S-adenosyl-L-methionine By similarity
Binding site1441Substrate By similarity
Binding site1761Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0ACP5 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 00A03E61CD8CA2E2

FASTA23727,016
        10         20         30         40         50         60 
MERRADAATG KRPIRSFVRR EGRLTAGQQR ALELLWPAFG LERPPAGQPL DLDRAFGRRA 

        70         80         90        100        110        120 
PRILEIGFGN GESLAEQAAT HPERDYLGIE VHRPGVGHLL MEVEKRHLGN VRVMMADAAE 

       130        140        150        160        170        180 
VLAHHIPDGS LHGVQLFFPD PWPKKRHHKR RLVQPQWVRA VAAKLAPGGF LHLATDWADY 

       190        200        210        220        230 
AEHMLDVLEA EPDLENTCGP RQFSPRGERP ETKFERRGLR KGHQVFDLYY RKREHPG 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000453 Genomic DNA. Translation: ABI55392.1.
RefSeqYP_740882.1. NC_008340.1.

3D structure databases

ProteinModelPortalQ0ACP5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187272.Mlg_0033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI55392; ABI55392; Mlg_0033.
GeneID4268890.
KEGGaeh:Mlg_0033.
PATRIC20859236. VBIAlkEhr114327_0033.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMALDKDHTI.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycAEHR187272:GHAX-33-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ALKEH
AccessionPrimary (citable) accession number: Q0ACP5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways