Reviewed, UniProtKB/Swiss-Prot Q0ACP5 (TRMB_ALHEH)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Mlg_0033

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000288116
Regions
	Region	212 – 215	4	Substrate binding Potential
Sites
	Binding site	65	1	S-adenosyl-L-methionine By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	117	1	S-adenosyl-L-methionine By similarity
	Binding site	140	1	S-adenosyl-L-methionine By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	176	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0ACP5-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 00A03E61CD8CA2E2	FASTA	237	27,016
	10 20 30 40 50 60 MERRADAATG KRPIRSFVRR EGRLTAGQQR ALELLWPAFG LERPPAGQPL DLDRAFGRRA 70 80 90 100 110 120 PRILEIGFGN GESLAEQAAT HPERDYLGIE VHRPGVGHLL MEVEKRHLGN VRVMMADAAE 130 140 150 160 170 180 VLAHHIPDGS LHGVQLFFPD PWPKKRHHKR RLVQPQWVRA VAAKLAPGGF LHLATDWADY 190 200 210 220 230 AEHMLDVLEA EPDLENTCGP RQFSPRGERP ETKFERRGLR KGHQVFDLYY RKREHPG

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI55392.1.
RefSeq	YP_740882.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4268890.
GenomeReviews	Gene locus Mlg_0033 in contig CP000453_GR.
KEGG	aeh:Mlg_0033.
NMPDR	fig|187272.6.peg.32.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0ACP5.
OMA	Q0ACP5. TKFENRG.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_ALHEH

Accession

Primary (citable) accession number: Q0ACP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)