ID   TYSY_ALHEH              Reviewed;         264 AA.
AC   Q0ABR1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=Thymidylate synthase;
DE            Short=TS;
DE            Short=TSase;
DE            EC=2.1.1.45;
GN   Name=thyA; OrderedLocusNames=Mlg_0371;
OS   Alkalilimnicola ehrlichei (strain MLHE-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Alkalilimnicola.
OX   NCBI_TaxID=187272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T.,
RA   King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T.,
RA   Xie G., Stolz J.F., Richardson P.;
RT   "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Provides the sole de novo source of dTMP for DNA
CC       biosynthesis (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP =
CC       dihydrofolate + dTMP.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Bacterial-
CC       type thyA subfamily.
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DR   EMBL; CP000453; ABI55726.1; -; Genomic_DNA.
DR   RefSeq; YP_741216.1; -.
DR   GeneID; 4268962; -.
DR   GenomeReviews; CP000453_GR; Mlg_0371.
DR   KEGG; aeh:Mlg_0371; -.
DR   NMPDR; fig|187272.6.peg.354; -.
DR   HOGENOM; Q0ABR1; -.
DR   OMA; Q0ABR1; LGPVYGH.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:HAMAP.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00008; -; 1.
DR   InterPro; IPR000398; Thymidylat_synth_C.
DR   Gene3D; G3DSA:3.30.572.10; Thymidylat_synth_C; 1.
DR   PANTHER; PTHR11549:SF2; Thymidylat_synth_C; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PRINTS; PR00108; THYMDSNTHASE.
DR   ProDom; PD001180; Thymidylat_synth; 1.
DR   TIGRFAMs; TIGR03284; thym_sym; 1.
DR   PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Methyltransferase;
KW   Nucleotide biosynthesis; Transferase.
FT   CHAIN         1    264       Thymidylate synthase.
FT                                /FTId=PRO_1000000572.
FT   ACT_SITE    146    146       By similarity.
SQ   SEQUENCE   264 AA;  30239 MW;  9A4A23669A89A258 CRC64;
     MQAYLDLMAH VRHHGTPKSD RTGTGTLSVF GHQMRFDLSR GFPVVTTKKL HLRSIIHELL
     WFLSGDSNIA YLKANGVSIW DEWADENGDL GPIYGVQWRS WPTPDGRRVD QLAQVLRDLR
     EHPDSRRHLV SAWNVGELPN MALPPCHTLF QFYVAEGRLS CQLYQRSADI FLGLPFNIAS
     YALLTHMVAQ VCDLKPGEFI WTGGDCHLYT NHLEQADRQL QREPLPLPRL RLNPEVRDLF
     AFRYEDIALE GYQHHPPIKA PVAV
//