Reviewed, UniProtKB/Swiss-Prot Q0ABN3 (SYL_ALHEH)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	Mlg_0400

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm. HAMAP MF_00049
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 864	864	Leucyl-tRNA synthetase HAMAP MF_00049		PRO_1000009288
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00049
	Motif	621 – 625	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	624	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0ABN3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 49BADF5A7AD2469E	FASTA	864	96,603
	10 20 30 40 50 60 MDASYQPEQI EAQAQRYWDE QQSFAAREER EGEKFYCLSM FPYPSGRLHM GHVRNYTIGD 70 80 90 100 110 120 VISRYQRMQG RNVLQPMGWD AFGLPAENAA MKHGVPPARW TRENIETMRG QLQRLGFAYD 130 140 150 160 170 180 WDREFATCDP EYYRWEQWLF TRLYKKGLVY KKTATVNWDP VDQTVLANEQ VVDGRGWRSG 190 200 210 220 230 240 AVVERRDIPQ WFLRITDYAD ELLEALDELP GWPEQVRAMQ RNWIGRSEGV ELDFAVAGHS 250 260 270 280 290 300 DTLRVYTTRP DTLYGVTYVG LAPEHPLAAE AAEGNPEMQR FIEDCRKGGV AEADIATMEK 310 320 330 340 350 360 RGMDTGLKAV HPLTGEQVPI WVANFVLMEY GHGAVMAVPA HDQRDWEFAS KYGLDIRPVV 370 380 390 400 410 420 HPSAEERADV SEGAFTDDGV LADSGEFSGL PSAEARQAIG EKLEGLGKGE RTVNYRLRDW 430 440 450 460 470 480 GISRQRYWGA PIPMIQCPAC GDVPVPDEDL PVVLPEDVDV TGGGSPLKDL PAFYQTTCPQ 490 500 510 520 530 540 CGGEARRETD TFDTFMESSW YYARFACADQ KGAMLDERAD QWLPVDQYIG GIEHAILHLL 550 560 570 580 590 600 YARFFHKLMR DEGLLQSDEP FRNLLTQGMV IAETYYRERG DGGKDWYNPA EVTVQRDERG 610 620 630 640 650 660 RPVSAVLEDD GEPVVMGAIE KMSKSKNNGV DPQALIDRYG ADTVRLYTMF AAPPDQSLEW 670 680 690 700 710 720 SDSAVEGAYR FLRRYYGLVR DHVAAGPVPA LDVASLDDAA RDLRRKVHET IAKASDDVGR 730 740 750 760 770 780 RYTFNTAIAA VMELCNALGK AAANEPSGAA RAVLQEGLEA ATLILAPIAP HVTHVCWQAL 790 800 810 820 830 840 GHDEAVIDAR WPAVDESALT RDTIELVVQV NGKLRSRLQL PADADKAAAE AAALADEKVQ 850 860 RFTEGKTVRK VIVVPGKLVN IVAN

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI55754.1.
RefSeq	YP_741244.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4269978.
GenomeReviews	Gene locus Mlg_0400 in contig CP000453_GR.
KEGG	aeh:Mlg_0400.
NMPDR	fig|187272.6.peg.378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0ABN3.
OMA	Q0ABN3. MMFASPP.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_ALHEH

Accession

Primary (citable) accession number: Q0ABN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)