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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei624 – 6241ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAEHR187272:GHAX-407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mlg_0400
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
ProteomesiUP000001962: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Leucine--tRNA ligasePRO_1000009288Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187272.Mlg_0400.

Structurei

3D structure databases

ProteinModelPortaliQ0ABN3.
SMRiQ0ABN3. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi621 – 6255"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0ABN3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDASYQPEQI EAQAQRYWDE QQSFAAREER EGEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMQG RNVLQPMGWD AFGLPAENAA MKHGVPPARW
110 120 130 140 150
TRENIETMRG QLQRLGFAYD WDREFATCDP EYYRWEQWLF TRLYKKGLVY
160 170 180 190 200
KKTATVNWDP VDQTVLANEQ VVDGRGWRSG AVVERRDIPQ WFLRITDYAD
210 220 230 240 250
ELLEALDELP GWPEQVRAMQ RNWIGRSEGV ELDFAVAGHS DTLRVYTTRP
260 270 280 290 300
DTLYGVTYVG LAPEHPLAAE AAEGNPEMQR FIEDCRKGGV AEADIATMEK
310 320 330 340 350
RGMDTGLKAV HPLTGEQVPI WVANFVLMEY GHGAVMAVPA HDQRDWEFAS
360 370 380 390 400
KYGLDIRPVV HPSAEERADV SEGAFTDDGV LADSGEFSGL PSAEARQAIG
410 420 430 440 450
EKLEGLGKGE RTVNYRLRDW GISRQRYWGA PIPMIQCPAC GDVPVPDEDL
460 470 480 490 500
PVVLPEDVDV TGGGSPLKDL PAFYQTTCPQ CGGEARRETD TFDTFMESSW
510 520 530 540 550
YYARFACADQ KGAMLDERAD QWLPVDQYIG GIEHAILHLL YARFFHKLMR
560 570 580 590 600
DEGLLQSDEP FRNLLTQGMV IAETYYRERG DGGKDWYNPA EVTVQRDERG
610 620 630 640 650
RPVSAVLEDD GEPVVMGAIE KMSKSKNNGV DPQALIDRYG ADTVRLYTMF
660 670 680 690 700
AAPPDQSLEW SDSAVEGAYR FLRRYYGLVR DHVAAGPVPA LDVASLDDAA
710 720 730 740 750
RDLRRKVHET IAKASDDVGR RYTFNTAIAA VMELCNALGK AAANEPSGAA
760 770 780 790 800
RAVLQEGLEA ATLILAPIAP HVTHVCWQAL GHDEAVIDAR WPAVDESALT
810 820 830 840 850
RDTIELVVQV NGKLRSRLQL PADADKAAAE AAALADEKVQ RFTEGKTVRK
860
VIVVPGKLVN IVAN
Length:864
Mass (Da):96,603
Last modified:October 17, 2006 - v1
Checksum:i49BADF5A7AD2469E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI55754.1.
RefSeqiYP_741244.1. NC_008340.1.

Genome annotation databases

EnsemblBacteriaiABI55754; ABI55754; Mlg_0400.
GeneIDi4269978.
KEGGiaeh:Mlg_0400.
PATRICi20859966. VBIAlkEhr114327_0390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI55754.1.
RefSeqiYP_741244.1. NC_008340.1.

3D structure databases

ProteinModelPortaliQ0ABN3.
SMRiQ0ABN3. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187272.Mlg_0400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI55754; ABI55754; Mlg_0400.
GeneIDi4269978.
KEGGiaeh:Mlg_0400.
PATRICi20859966. VBIAlkEhr114327_0390.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAEHR187272:GHAX-407-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1101 / DSM 17681 / MLHE-1.

Entry informationi

Entry nameiSYL_ALKEH
AccessioniPrimary (citable) accession number: Q0ABN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.