Reviewed, UniProtKB/Swiss-Prot Q0ABN0 (PROA_ALHEH)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Mlg_0403

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000340870

Sequences

	Sequence		Length	Mass (Da)
	Q0ABN0-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A74B5C5EE21FC49A	FASTA	420	45,465
	10 20 30 40 50 60 MTEDIRAYMQ TLGRQAREAA REMARADTGH KNAALHAMAD ALEEHAGDLK RANADDLENG 70 80 90 100 110 120 RANNLDPALL DRLELNDERI HGMAEGLRQI AALPDPVGAI RDLAFRPSGI QVGRMRVPLG 130 140 150 160 170 180 VIGIIYESRP NVTADAAALC LKSGNAAILR GGSEARHSNA AIAACVRAGL KRAGLPGEAV 190 200 210 220 230 240 QVVETTDRAA VGELIRMDEY VDVLVPRGGK GLIERITAEA TVPVIKHLDG ICHVYIDDSA 250 260 270 280 290 300 DPAKAISVAY NAKTQRLGTC NTMETLLVAE AIAERVLPEL ARRYRESEVE LRGCEATRRL 310 320 330 340 350 360 IPDAVPATKE DWSTEYLDAI VSIRIVPGLD EAIEHIARHS SGHTESILTE DWSRARRFLR 370 380 390 400 410 420 EVDSSSVMVN ASTRFADGFE YGLGAEIGIS TDKLHARGPV GLEGLTTEKF IVLGDGHIRE

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI55757.1.
RefSeq	YP_741247.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0ABN0.
Genome annotation databases
GeneID	4269981.
GenomeReviews	Gene locus Mlg_0403 in contig CP000453_GR.
KEGG	aeh:Mlg_0403.
NMPDR	fig|187272.6.peg.381.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0ABN0.
OMA	FDTEWLD.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ALHEH

Accession

Primary (citable) accession number: Q0ABN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)