Reviewed, UniProtKB/Swiss-Prot Q0AB94 (SYP_ALHEH)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mlg_0539

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000288308

Sequences

	Sequence		Length	Mass (Da)
	Q0AB94-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 49D836E1F1B5FF48	FASTA	568	62,591
	10 20 30 40 50 60 MRASLFPLST SKETPADAEI VSHQLMLRAG MIRKLAAGLY TWTPLGLRVL RKVEQIVREE 70 80 90 100 110 120 MDRAGAHELL MPAVQPAELW QESTRWDKYG PELLRLKDRH ERDFCFGPTH EEVITDYVRR 130 140 150 160 170 180 EVKSYRQLPL NLYQIQTKFR DEIRPRFGVM RAREFLMKDA YSFHLDDDCL ARTYQVMYET 190 200 210 220 230 240 YTRIFERTGL VFRAVAADSG NIGGSVSHEF HVLAESGEDA VAFSDESDYA ANVELAEAVA 250 260 270 280 290 300 PAGEAPPPAE TMRRVDTPGA RTIDDLVRDY GLPIEKTVKT LVVHGADGGL VALLVRGDHS 310 320 330 340 350 360 LNDVKATTLP QVAEPLVMAG EEEIRAAVGA GPGSLGPVEL PLPCVVDRSV AVMSDFAAGA 370 380 390 400 410 420 NQDDAHYFGI NWGRDVALPE VADLREVVAG DPSPDGRGTL EIARGIEVGH IFQLGREYSE 430 440 450 460 470 480 KMKATVLNEA GDAQTVTMGC YGIGVSRVVA AAIEQNHDDN GIIWPAPIAP FQLALVPIGM 490 500 510 520 530 540 NRSEAVTEQA EKLYAELQAE GVEVFFDDRD ARPGVKFADM ELIGIPHRLV IGDRGLKNGV 550 560 VEYRGRRDSE STDVPLAELS AFLRERLG

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI55893.1.
RefSeq	YP_741383.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4268068.
GenomeReviews	Gene locus Mlg_0539 in contig CP000453_GR.
KEGG	aeh:Mlg_0539.
NMPDR	fig|187272.6.peg.512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AB94.
OMA	Q0AB94. VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ALHEH

Accession

Primary (citable) accession number: Q0AB94

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)