Reviewed, UniProtKB/Swiss-Prot Q0AB75 (AMPA_ALHEH)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Mlg_0558

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019877
Sites
	Active site	280	1	Potential
	Active site	354	1	Potential
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	273	1	Manganese 1 By similarity
	Metal binding	273	1	Manganese 2 By similarity
	Metal binding	291	1	Manganese 2 By similarity
	Metal binding	350	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AB75-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 5AAD5C4C88A3B868	FASTA	497	52,971
	10 20 30 40 50 60 MDHTVKSKTA DTVSSPCAVV GVFERRRLSP AAKAVDEASG GAITAALKRG DIEAKPGQTR 70 80 90 100 110 120 LLTDLDNVKA ARVLLVGLGV ERDLDERTYR KAVTAAAQAA QDCGAGEATF YLPEVEVKTR 130 140 150 160 170 180 DLAWRVQQLA IGVTGALYRF DDMKSEAETP RKPLKKLALG VADKAEAKVA DEALAQGMAV 190 200 210 220 230 240 GRGMSLARDL GNLPGNVCTP SYLADQAKAL GKRFDKLKVQ SLDRKDMKKL GMGALLAVAQ 250 260 270 280 290 300 GSQEEPRLIA MEWNGGKKDE QPYVLVGKGI TFDTGGISLK PGAAMDEMKF DMCGAASVFG 310 320 330 340 350 360 TLQAVAEMNL PINVVGVVPA SDNMPDGKAT RPGDIIQTLS GQTVEVLNTD AEGRLVLCDA 370 380 390 400 410 420 LTWSERFKPK EIVDIATLTG ACIIALGHHR SAVLGNHPPL VKALQDAGET SGDKCWELPL 430 440 450 460 470 480 DPEYDEQLKS NFADMANIGG RPAGTITAAS FLARFTKRYQ WAHLDIAGTA WLTGEQKGAT 490 GRPVPLLTRY LMDRAGA

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI55912.1.
RefSeq	YP_741402.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AB75.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	4270313.
GenomeReviews	Gene locus Mlg_0558 in contig CP000453_GR.
KEGG	aeh:Mlg_0558.
NMPDR	fig|187272.6.peg.531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AB75.
OMA	LGHHNSG.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ALHEH

Accession

Primary (citable) accession number: Q0AB75

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)