Reviewed, UniProtKB/Swiss-Prot Q0AB36 (PYRC_ALHEH)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Mlg_0597

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Dihydroorotase		PRO_0000325568
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AB36-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 62C22D3DAED42972	FASTA	343	37,531
	10 20 30 40 50 60 MQRITLTRPD DWHLHLRDGE QLATVLPHTA TVFGRAIIMP NLKPPVTTVD QAAAYRERIL 70 80 90 100 110 120 AALPAGAHFE PLMTLYLTDN TPPAEIEKAA ASGFVHAVKL YPAGATTNSD AGVTDLARCE 130 140 150 160 170 180 ETLAAMAERG LPLCVHGEVT RDEVDIFDRE AHFIDEVLDP LVQRHSRLRV VFEHITTQAA 190 200 210 220 230 240 VDYLRQAPDR VGATLTVQHL MANRNHMLVG GVRPHYYCLP ILKRERDRQA LVEAATSGHP 250 260 270 280 290 300 RFFLGTDSAP HPKGAKESAC GCAGVYSAHA ALPFYAEIFE AAGALDRLEG FASHHGADFY 310 320 330 340 GLPRNRDSVT LERAATPIPE AFPMGDDTLV PFRAGGEVAW RVV

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI55951.1.
RefSeq	YP_741441.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4270376.
GenomeReviews	Gene locus Mlg_0597 in contig CP000453_GR.
KEGG	aeh:Mlg_0597.
NMPDR	fig|187272.6.peg.570.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AB36.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ALHEH

Accession

Primary (citable) accession number: Q0AB36

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 17, 2006
Last modified:	November 25, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)