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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Thioimide intermediateUniRule annotation
Active sitei41 – 411Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciAEHR187272:GHAX-704-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:Mlg_0678
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
Proteomesi
  • UP000001962 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 129129NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000062374Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187272.Mlg_0678.

Structurei

3D structure databases

ProteinModelPortaliQ0AAV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni56 – 583Substrate bindingUniRule annotation
Regioni75 – 762Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107YRI. Bacteria.
COG0780. LUCA.
HOGENOMiHOG000009528.
KOiK09457.
OMAiLYINSYR.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00818. QueF_type1.
InterProiIPR029500. QueF.
IPR016856. QueF_type1.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
[Graphical view]
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0AAV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQPSKDLE TFPNPRPERD FVLHMRIPEF TCLCPKTGQP DFATIHLDYV
60 70 80 90 100
PDERCVELKS LKLYMWSFRD QGAFHEAITN EILDDLVRAT EPRYMKVTAE
110 120
FYVRGGIYTT VVAEHRKPGW APAPKVELA
Length:129
Mass (Da):14,915
Last modified:October 17, 2006 - v1
Checksum:i9ED07706B4458CB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI56032.1.
RefSeqiWP_011628427.1. NC_008340.1.

Genome annotation databases

EnsemblBacteriaiABI56032; ABI56032; Mlg_0678.
KEGGiaeh:Mlg_0678.
PATRICi20860568. VBIAlkEhr114327_0672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI56032.1.
RefSeqiWP_011628427.1. NC_008340.1.

3D structure databases

ProteinModelPortaliQ0AAV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187272.Mlg_0678.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI56032; ABI56032; Mlg_0678.
KEGGiaeh:Mlg_0678.
PATRICi20860568. VBIAlkEhr114327_0672.

Phylogenomic databases

eggNOGiENOG4107YRI. Bacteria.
COG0780. LUCA.
HOGENOMiHOG000009528.
KOiK09457.
OMAiLYINSYR.
OrthoDBiEOG6D5G0M.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciAEHR187272:GHAX-704-MONOMER.

Family and domain databases

HAMAPiMF_00818. QueF_type1.
InterProiIPR029500. QueF.
IPR016856. QueF_type1.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
[Graphical view]
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1101 / DSM 17681 / MLHE-1.

Entry informationi

Entry nameiQUEF_ALKEH
AccessioniPrimary (citable) accession number: Q0AAV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 17, 2006
Last modified: December 9, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.