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Q0AAV2 (DNLJ_ALHEH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:Mlg_0681
OrganismAlkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]
Taxonomic identifier187272 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length673 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 673673DNA ligase HAMAP MF_01588
PRO_0000313108

Regions

Domain592 – 67382BRCT
Nucleotide binding36 – 405NAD By similarity
Nucleotide binding85 – 862NAD By similarity

Sites

Active site1181N6-AMP-lysine intermediate By similarity
Metal binding4091Zinc By similarity
Metal binding4121Zinc By similarity
Metal binding4271Zinc By similarity
Metal binding4331Zinc By similarity
Binding site1161NAD By similarity
Binding site1391NAD By similarity
Binding site1761NAD By similarity
Binding site2911NAD By similarity
Binding site3151NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0AAV2 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 66111D6D190F6897

FASTA67373,796
        10         20         30         40         50         60 
MAQTGTDPQA RIEALRREIR EHDHRYYVLD APVIADAEYD ALMAELQALE AEHPELITPD 

        70         80         90        100        110        120 
SPSQRVAGRP AEGFGEVTHA EPMLSLDNAF EEADLAEFDR RVRQALGLDP VVYVAEPKLD 

       130        140        150        160        170        180 
GLSVSIRYED GRLVRAGTRG DGRVGEAITE NVRTIRSVPL RLRGEGWPPV MEVRGEVVIR 

       190        200        210        220        230        240 
RADFERLNEQ RLADGERPFA NPRNAAAGSL RQLDPRITAR RRLTFFTFGV AAAGRLAASH 

       250        260        270        280        290        300 
HEVLDKLAGW GFRVNERVER VRGLDGCREY YQRLLADRDE LSFEIDGVVY KVDDLDAREE 

       310        320        330        340        350        360 
LGFTARAPRW AIAWKLPAQE ATTVVRRILP SVGRTGAITP VAELEPVGVG GVTVSRATLH 

       370        380        390        400        410        420 
NLDEVRRKDV RKGDTVMVRR AGDVIPEITA VVTEKRPEGA EPWAMPAECP VCGSEVLRLD 

       430        440        450        460        470        480 
DEAVHRCMGG LYCPAQREGA LLHFASRKAL DIDGLGEKVV SQLVERGMVR SPADLFTLEH 

       490        500        510        520        530        540 
CQLAGLERMG DKSADNLVAA LDKARRTTLP RFLYALGIQH VGEVTARRLA EHFGSLEAIM 

       550        560        570        580        590        600 
NADESALAET PDVGPVVAQA IAHFFAEPHN REVVQALRAA GVTWEEVDPA ERGEQPLAGR 

       610        620        630        640        650        660 
TFVLTGTLSG MTRDEAKAAL EALGARVSGS VSKKTDYLVA GEKAGSKLAK AESLGVEVLD 

       670 
EQALQALLQE HGR

« Hide

References

[1]"Complete sequence of Alkalilimnicola ehrilichei MLHE-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MLHE-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000453 Genomic DNA. Translation: ABI56035.1.
RefSeqYP_741525.1. NC_008340.1.

3D structure databases

ProteinModelPortalQ0AAV2.
SMRQ0AAV2. Positions 7-586.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0AAV2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4268476.
GenomeReviewsGene locus Mlg_0681 in contig CP000453_GR.
KEGGaeh:Mlg_0681.
NMPDRfig|187272.6.peg.652.
PATRIC20860574. VBIAlkEhr114327_0675.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0272.
HOGENOMHBG620317.
OMAENVRTIR.
PhylomeDBQ0AAV2.
ProtClustDBCLSK2516571.

Enzyme and pathway databases

BioCycAEHR187272:MLG_0681-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 4 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. False negative.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_ALHEH
AccessionPrimary (citable) accession number: Q0AAV2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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