Reviewed, UniProtKB/Swiss-Prot Q0AAL5 (SYT_ALHEH)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Mlg_0768

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000020335
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AAL5-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 0024C284EDA6ADA7	FASTA	641	73,783
	10 20 30 40 50 60 MPAITLPDGS RREFDHPVTV RDVAESIGKG LARAALAGRV DGRLVDLSHR IDDDVQLEIV 70 80 90 100 110 120 TDRDPDGVDM IRHSTAHLMA QAVKELFPEA QVTIGPVIEN GFYYDFAYKR PFTEDDLKAI 130 140 150 160 170 180 EKKMEELARQ DFEVHREVWD RDEAIRFFLE QGEQYKAEII EDLPEHETIS VYKQGDFLDL 190 200 210 220 230 240 CRGPHVPNTG KLKAFKLQKV AGAYWRGKSD NEMLQRIYGT AWPDKKQLKA YLHRLAEAEK 250 260 270 280 290 300 RDHRRIGRAQ NLFHLQEEAP GMAFWHPKGW QIYLEVQEYI RRLTRGHGYQ EIHTPQLVDR 310 320 330 340 350 360 SLWEKSGHWD KFQDNMFITE SESRDYAVKP MNCPCHVQVY NQGLKSYRDL PLRLAEFGSC 370 380 390 400 410 420 HRNEPSGTLH GLMRVRNFVQ DDAHIFCTED QIQQEVADFI DLVFKAYNDF GFDDVIIALS 430 440 450 460 470 480 TRPDERVGED ALWDKAEHAL EEALNRAGLE WQLQPGEGAF YGPKIEFTLK DCLGRTWQLG 490 500 510 520 530 540 TIQVDFSMPG RLGATYVAED GSKKVPVMLH RAILGSLERF IGILIEHYAG ALPPWLAPVQ 550 560 570 580 590 600 AVVLNITDRQ GEYARRIEKR FRERGLRVDA DLRNEKIGFK IREHTLQKVP YMLVVGDKEV 610 620 630 640 ENNTVAVRTR EGQDLGSMAV DDFLQRLESE IARLGRSNSE D

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI56122.1.
RefSeq	YP_741612.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AAL5.
Genome annotation databases
GeneID	4268582.
GenomeReviews	Gene locus Mlg_0768 in contig CP000453_GR.
KEGG	aeh:Mlg_0768.
NMPDR	fig|187272.6.peg.737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AAL5.
OMA	QDEAPGM.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ALHEH

Accession

Primary (citable) accession number: Q0AAL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)