Reviewed, UniProtKB/Swiss-Prot Q0AAG6 (SYM_ALHEH)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Mlg_0817

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 675	675	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331777
Regions
	Domain	574 – 675	102	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	328 – 332	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	331	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AAG6-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: F7E4C82EEDE1A7AC	FASTA	675	76,942
	10 20 30 40 50 60 MTRKILVTSA LPYANGPIHL GHLVEYIQTD IWARFQRLRG HDCHYVCADD AHGTPIMLKA 70 80 90 100 110 120 RERGITPEAL IAEVGEEHQR DFADFLIEFD NYHTTHSDEN RYFAELIYSR LNQAGHIDRK 130 140 150 160 170 180 VIKQAYDPKL EMFLPDRYIK GECPRCGAED QYGDSCEACG ATYTPAELKN AVSVVSGERP 190 200 210 220 230 240 VERESEHYFF RLQDFEAMLR EWASPEHLQA EVANKLAEWF KEGLRSWDIS RDAPYFGFRI 250 260 270 280 290 300 PDTEDKYFYV WLDAPIGYMA SFKHLCERKG LDFDDWWGPD STAELYHFIG KDIIYFHALF 310 320 330 340 350 360 WPAMLHGAGF RKPTEICAHG FLTVNGQKMS KSRGTFIMAR TWLDHLNPEY LRYYFAAKLS 370 380 390 400 410 420 ASVHDLDLSL DDFTQRVNAD LVGKLVNIAS RCAGFIHKRF EGRLGETLDD PALYRQFTAA 430 440 450 460 470 480 GEHIAELYEK REFSRAMREI MALADQANQY VDEQKPWVLA KQEDQADRVQ DICTQGLNLF 490 500 510 520 530 540 RVLVVYLKPV LPRLAADAEA FLNLPEQCWA DAKTPLLDHG IRKFKPLMTR VDPDRVQAML 550 560 570 580 590 600 EDSKKTLQPK GEPQAAEAGP LTSDPIAEQI EIGDFAKVDL RIARIEQAEH VEGADKLLRL 610 620 630 640 650 660 QLDLGGETRQ VFAGIKQAYR PEDLEGRLTV MAANLKPRKM KFGVSEGMVL AAGPGGSDLY 670 LLEPHQGAQP GMRVK

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI56171.1.
RefSeq	YP_741661.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AAG6.
Genome annotation databases
GeneID	4270648.
GenomeReviews	Gene locus Mlg_0817 in contig CP000453_GR.
KEGG	aeh:Mlg_0817.
NMPDR	fig|187272.6.peg.787.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AAG6.
OMA	SAYQPEQ.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ALHEH

Accession

Primary (citable) accession number: Q0AAG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)