Reviewed, UniProtKB/Swiss-Prot Q0AAD1 (SYI_ALHEH)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	Mlg_0852

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	941 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 941	941	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_1000022040
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP MF_02002
	Motif	603 – 607	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	904	1	Zinc By similarity
	Metal binding	907	1	Zinc By similarity
	Metal binding	924	1	Zinc By similarity
	Metal binding	927	1	Zinc By similarity
	Binding site	562	1	Aminoacyl-adenylate By similarity
	Binding site	606	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AAD1-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 2B8F9AE6731EAC86	FASTA	941	105,903
	10 20 30 40 50 60 MSDYKHTLNL PKTGFPMRGN LAKREPERLA GWYQTDLYGR LRRERAGKPR FVLHDGPPYA 70 80 90 100 110 120 NGDIHIGHAV NKILKDIIIK ARSMDGYDVP YVPGWDCHGL PIELMVEKKR GKAGAKVSPR 130 140 150 160 170 180 AFRDACREFA ASQVDGQRED FKRLGVLGDW DNPYLTMDYR TEADILRALG RIIQRGHVTR 190 200 210 220 230 240 GFKPVHWCAD CGSALAEAEV EYEEKTSPAI DVRFAVLEPE ELDRRAGLGG EAAAAGRVAI 250 260 270 280 290 300 PIWTTTPWTL PANQAVALHP ELEYVVVAFD DELLVLAAEL VESAMARYEV DDYRVVGRCD 310 320 330 340 350 360 GAVLEGLRLA HPFLEREVPV ILGGHVTTDG GTGAVHTAPG HGQDDYVVGQ QYDLPTDNPV 370 380 390 400 410 420 DGNGVFLPDT PFFAGQHVFK ANPKVVDLLA ERGALLHHEP YRHSYPHCWR HKTPILFRAT 430 440 450 460 470 480 PQWFISLDKA GMREHAMAAI KGVSWHPEWG QARIESMVNG RPDWCISRQR NWGVPIALFV 490 500 510 520 530 540 DKRSGEPHPE SERLIEAVAR RVEEAGVDAW FELDPAELLG ADAERYEKVT DILDVWFDSG 550 560 570 580 590 600 VTHATVLERR DELQVPADLY LEGSDQHRGW FQSSLLTSVG VRETAPYKGV LTHGFTVDEK 610 620 630 640 650 660 GHKMSKSRGN VVAPQKVMDT LGADILRLWV ASSDYSAEMA VSDGILKRTA DAYRRMRNTA 670 680 690 700 710 720 RFLLANLNGF EPAEHAVAPP DMLPLDRWAV DRAYLLQQQV REAYERYEFH RIYQMVHNFC 730 740 750 760 770 780 VVDLGGFYLD VIKDRQYTTK PDSLARRSCQ TALWHVAEGL VRWLAPIISF TAEEIWEHLP 790 800 810 820 830 840 GERSDSVLLE TWYEGLFPLD DSDPFGRAFW DDVLAVRAGV NRELEQLRND KVIGASLQAE 850 860 870 880 890 900 VQLFCPPELK AKLDRLGDEL RFVLITSEAR VEDLERAPVE SVEVPGENGQ GFRLFAAASQ 910 920 930 940 HPKCTRCWHH RPDVGHHADH PELCGRCVSN VDGEGETRHY A

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI56206.1.
RefSeq	YP_741696.1.
3D structure databases
SMR	Q0AAD1. Positions 3-932.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AAD1.
Genome annotation databases
GeneID	4270789.
GenomeReviews	Gene locus Mlg_0852 in contig CP000453_GR.
KEGG	aeh:Mlg_0852.
NMPDR	fig|187272.6.peg.822.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HBG577712.
OMA	KQVLTHG.
PhylomeDB	Q0AAD1.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_ALHEH

Accession

Primary (citable) accession number: Q0AAD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)