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Q0AAD1

- SYI_ALKEH

UniProt

Q0AAD1 - SYI_ALKEH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
Binding sitei606 – 6061ATPUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAEHR187272:GHAX-881-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mlg_0852
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
ProteomesiUP000001962: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligasePRO_1000022040Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187272.Mlg_0852.

Structurei

3D structure databases

ProteinModelPortaliQ0AAD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi603 – 6075"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0AAD1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PKTGFPMRGN LAKREPERLA GWYQTDLYGR LRRERAGKPR
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAV NKILKDIIIK ARSMDGYDVP YVPGWDCHGL
110 120 130 140 150
PIELMVEKKR GKAGAKVSPR AFRDACREFA ASQVDGQRED FKRLGVLGDW
160 170 180 190 200
DNPYLTMDYR TEADILRALG RIIQRGHVTR GFKPVHWCAD CGSALAEAEV
210 220 230 240 250
EYEEKTSPAI DVRFAVLEPE ELDRRAGLGG EAAAAGRVAI PIWTTTPWTL
260 270 280 290 300
PANQAVALHP ELEYVVVAFD DELLVLAAEL VESAMARYEV DDYRVVGRCD
310 320 330 340 350
GAVLEGLRLA HPFLEREVPV ILGGHVTTDG GTGAVHTAPG HGQDDYVVGQ
360 370 380 390 400
QYDLPTDNPV DGNGVFLPDT PFFAGQHVFK ANPKVVDLLA ERGALLHHEP
410 420 430 440 450
YRHSYPHCWR HKTPILFRAT PQWFISLDKA GMREHAMAAI KGVSWHPEWG
460 470 480 490 500
QARIESMVNG RPDWCISRQR NWGVPIALFV DKRSGEPHPE SERLIEAVAR
510 520 530 540 550
RVEEAGVDAW FELDPAELLG ADAERYEKVT DILDVWFDSG VTHATVLERR
560 570 580 590 600
DELQVPADLY LEGSDQHRGW FQSSLLTSVG VRETAPYKGV LTHGFTVDEK
610 620 630 640 650
GHKMSKSRGN VVAPQKVMDT LGADILRLWV ASSDYSAEMA VSDGILKRTA
660 670 680 690 700
DAYRRMRNTA RFLLANLNGF EPAEHAVAPP DMLPLDRWAV DRAYLLQQQV
710 720 730 740 750
REAYERYEFH RIYQMVHNFC VVDLGGFYLD VIKDRQYTTK PDSLARRSCQ
760 770 780 790 800
TALWHVAEGL VRWLAPIISF TAEEIWEHLP GERSDSVLLE TWYEGLFPLD
810 820 830 840 850
DSDPFGRAFW DDVLAVRAGV NRELEQLRND KVIGASLQAE VQLFCPPELK
860 870 880 890 900
AKLDRLGDEL RFVLITSEAR VEDLERAPVE SVEVPGENGQ GFRLFAAASQ
910 920 930 940
HPKCTRCWHH RPDVGHHADH PELCGRCVSN VDGEGETRHY A
Length:941
Mass (Da):105,903
Last modified:October 17, 2006 - v1
Checksum:i2B8F9AE6731EAC86
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000453 Genomic DNA. Translation: ABI56206.1.
RefSeqiYP_741696.1. NC_008340.1.

Genome annotation databases

EnsemblBacteriaiABI56206; ABI56206; Mlg_0852.
GeneIDi4270789.
KEGGiaeh:Mlg_0852.
PATRICi20860933. VBIAlkEhr114327_0851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000453 Genomic DNA. Translation: ABI56206.1 .
RefSeqi YP_741696.1. NC_008340.1.

3D structure databases

ProteinModelPortali Q0AAD1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187272.Mlg_0852.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI56206 ; ABI56206 ; Mlg_0852 .
GeneIDi 4270789.
KEGGi aeh:Mlg_0852.
PATRICi 20860933. VBIAlkEhr114327_0851.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AEHR187272:GHAX-881-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1101 / DSM 17681 / MLHE-1.

Entry informationi

Entry nameiSYI_ALKEH
AccessioniPrimary (citable) accession number: Q0AAD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3