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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei368 – 3681NucleophileUniRule annotation
Active sitei421 – 4211Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciAEHR187272:GHAX-987-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Mlg_0958
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
ProteomesiUP000001962: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6956951,4-alpha-glucan branching enzyme GlgBPRO_0000260635Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187272.Mlg_0958.

Structurei

3D structure databases

ProteinModelPortaliQ0AA26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0AA26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVYDPHAEA VAFEDGTKLP RRPGSDFFEG PLPAGLSAPY RLRRTSQDGA
60 70 80 90 100
AHTAHDPYAF GPALPDYDLH LFGEGRHYHA QRFLGAHPLE HEGVPGVRFA
110 120 130 140 150
VWAPNAERVS VVGDFNRWDG RCHPMRVRGG SGVWELFIPG LPDGSLYKFE
160 170 180 190 200
LRNRDSGAVL VKTDPYGRRY QLRPDSAAVV EPPADYAWND REWMAARRED
210 220 230 240 250
QWQSIPISIY EVHLGSWQRA GDGSFLDYRT LAHRLVDHVL ATGFTHIELL
260 270 280 290 300
PITEHPFDGS WGYQTTGYFA PTTRFGAPDD FRYFVDHCHR HGIGVILDWV
310 320 330 340 350
PAHFPRDDWA LANFDGTPLY EHADPRRGEH RDWGTLIFNF GRNEVRNFLT
360 370 380 390 400
ASALYWLEEF HIDGLRVDAV ASMLYLDYSR DEGDWVPNIH GGNENLEAID
410 420 430 440 450
FLQELNRITH GEQPGTMIMA EESTSWPQVT RPTWLGGLGF TMKWNMGWMH
460 470 480 490 500
DTLTYMGEDP IHRRYHHDQL TFGMLYAFTE NFVLPFSHDE VVHGKRSLLW
510 520 530 540 550
RMPGDEWQRF ANLRLLYAYQ FTYPGKKLLF MGCEFGQGNE WNAEAALDWY
560 570 580 590 600
VLDYPLHQGV LRLVGDLNAL YREVPALHRH DFDEHGFAWI DCHDTEQSAI
610 620 630 640 650
SYLRRDGGHD GEATPPAATV LNFTPVPRHD YRLGVPAPGY WRERLNTDST
660 670 680 690
HYGGTNLGNG GGVWAEAVPW MGHPWSVVLT LPPLAGVVLS PEAGP
Length:695
Mass (Da):79,236
Last modified:October 17, 2006 - v1
Checksum:iAB4E1FFDFF96037F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI56311.1.
RefSeqiYP_741801.1. NC_008340.1.

Genome annotation databases

EnsemblBacteriaiABI56311; ABI56311; Mlg_0958.
GeneIDi4270428.
KEGGiaeh:Mlg_0958.
PATRICi20861143. VBIAlkEhr114327_0956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI56311.1.
RefSeqiYP_741801.1. NC_008340.1.

3D structure databases

ProteinModelPortaliQ0AA26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187272.Mlg_0958.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI56311; ABI56311; Mlg_0958.
GeneIDi4270428.
KEGGiaeh:Mlg_0958.
PATRICi20861143. VBIAlkEhr114327_0956.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciAEHR187272:GHAX-987-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1101 / DSM 17681 / MLHE-1.

Entry informationi

Entry nameiGLGB_ALKEH
AccessioniPrimary (citable) accession number: Q0AA26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 17, 2006
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.