Reviewed, UniProtKB/Swiss-Prot Q0AA26 (GLGB_ALHEH)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Mlg_0958

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000260635
Sites
	Active site	263	1	By similarity
	Active site	298	1	By similarity
	Active site	303	1	By similarity
	Active site	366	1	By similarity
	Active site	368	1	By similarity
	Active site	421	1	By similarity
	Active site	488	1	By similarity
	Active site	489	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AA26-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: AB4E1FFDFF96037F	FASTA	695	79,236
	10 20 30 40 50 60 MRVYDPHAEA VAFEDGTKLP RRPGSDFFEG PLPAGLSAPY RLRRTSQDGA AHTAHDPYAF 70 80 90 100 110 120 GPALPDYDLH LFGEGRHYHA QRFLGAHPLE HEGVPGVRFA VWAPNAERVS VVGDFNRWDG 130 140 150 160 170 180 RCHPMRVRGG SGVWELFIPG LPDGSLYKFE LRNRDSGAVL VKTDPYGRRY QLRPDSAAVV 190 200 210 220 230 240 EPPADYAWND REWMAARRED QWQSIPISIY EVHLGSWQRA GDGSFLDYRT LAHRLVDHVL 250 260 270 280 290 300 ATGFTHIELL PITEHPFDGS WGYQTTGYFA PTTRFGAPDD FRYFVDHCHR HGIGVILDWV 310 320 330 340 350 360 PAHFPRDDWA LANFDGTPLY EHADPRRGEH RDWGTLIFNF GRNEVRNFLT ASALYWLEEF 370 380 390 400 410 420 HIDGLRVDAV ASMLYLDYSR DEGDWVPNIH GGNENLEAID FLQELNRITH GEQPGTMIMA 430 440 450 460 470 480 EESTSWPQVT RPTWLGGLGF TMKWNMGWMH DTLTYMGEDP IHRRYHHDQL TFGMLYAFTE 490 500 510 520 530 540 NFVLPFSHDE VVHGKRSLLW RMPGDEWQRF ANLRLLYAYQ FTYPGKKLLF MGCEFGQGNE 550 560 570 580 590 600 WNAEAALDWY VLDYPLHQGV LRLVGDLNAL YREVPALHRH DFDEHGFAWI DCHDTEQSAI 610 620 630 640 650 660 SYLRRDGGHD GEATPPAATV LNFTPVPRHD YRLGVPAPGY WRERLNTDST HYGGTNLGNG 670 680 690 GGVWAEAVPW MGHPWSVVLT LPPLAGVVLS PEAGP

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI56311.1.
RefSeq	YP_741801.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AA26.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	4270428.
GenomeReviews	Gene locus Mlg_0958 in contig CP000453_GR.
KEGG	aeh:Mlg_0958.
NMPDR	fig|187272.6.peg.922.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0AA26.
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ALHEH

Accession

Primary (citable) accession number: Q0AA26

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)