Reviewed, UniProtKB/Swiss-Prot Q0A9G3 (DNAE2_ALHEH)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Mlg_1175

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070587

Sequences

	Sequence		Length	Mass (Da)
	Q0A9G3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: D87F812D48EFE07A	FASTA	1,025	114,101
	10 20 30 40 50 60 MPIDYAELHC LSCFSFLRGA SQPAELVQRA AELGYRALAL TDACSVAGAV RAHQAAKETD 70 80 90 100 110 120 LHLIHGSEIR IHQGPLLVLL APCRRAWAEL CALISLGRSQ ARKGDYRLER EQLEGTLPHC 130 140 150 160 170 180 LALWVPDDAP HDAEQGRWFA RHFGDRGHVA VALHHGPDDE ARLQRLLALA DRFRLPAVAA 190 200 210 220 230 240 GGVLMHRRGR RALQDTLSAL RHRRTLAAMG TALECSGERH LRSLHSLARL YPPALLRRSV 250 260 270 280 290 300 HLADQCRFSL DKLRYEYPAE LVPAGETPAS YLRRLTLEGA RRHWPQGMPD KVAHQVDHEL 310 320 330 340 350 360 ALIAEMGYEP FFLTVHDVVA FARRRGILCQ GRGSSANSAV CFCLGITAVD PARQSLLFER 370 380 390 400 410 420 FISKERGEPP DIDVDFEHER REEVIQYIYR KYGRHRAALA ATVIRYRPRS ALRDAGRALG 430 440 450 460 470 480 LDAATIDRLA GSIQWWDGKR VDPERLREAG LNPDDPRLAR TVAIAGQLLG LPRHLSQHVG 490 500 510 520 530 540 GFVISEGPIS ELVPTENAAM AGRTIIQWDK DDLEALGLLK VDVLALGMLS CIRRAFDLLA 550 560 570 580 590 600 GFRGRRLTLA DVPAEDPAVY RMISDADTMG VFQIESRAQM AMLPRLRPQT FYDLVIEVAI 610 620 630 640 650 660 VRPGPIQGDM VHPYLRRREG LEPVDYPSEA VRGVLARTLG VPIFQEQVMQ LAVVAAGFTP 670 680 690 700 710 720 GEADALRRAM AAWKRKGGLG PFRDKLLKGM RRNGYCEDYA ERLFRQIQGF GEYGFPESHA 730 740 750 760 770 780 ASFALLVYVS AWLKCHEPAL FTCALLNSQP MGFYAPAQLL RDAERHGVEI RPVDVRHSDW 790 800 810 820 830 840 DCSPEHRGDG EPALRLGLRL VRGLNRRAAD RLIAARGRRP FRDVQEMARR AALHRRDLET 850 860 870 880 890 900 LAHAGALRGL AGHRRAAWWQ VLGAEAGLPV FEDLHIEEAA PALDAPAEGE DLVADYTSLG 910 920 930 940 950 960 FTLGRHPLAL LRPQLRRRRL LTAADLASTG HGRLVRTAGL VINRQRPGSA GGVTFLTLED 970 980 990 1000 1010 1020 ETGQINLVVW KATAEAQRRT LLAARLLMVS GIWERKGAVT HLVAGRLEDW SDWLGALDVR SRDFH

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI56524.1.
RefSeq	YP_742014.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A9G3.
Genome annotation databases
GeneID	4269114.
GenomeReviews	Gene locus Mlg_1175 in contig CP000453_GR.
KEGG	aeh:Mlg_1175.
NMPDR	fig|187272.6.peg.1124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	LATIPQE.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_ALHEH

Accession

Primary (citable) accession number: Q0A9G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)