Reviewed, UniProtKB/Swiss-Prot Q0A923 (GLPK_ALHEH)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Mlg_1315

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Glycerol kinase HAMAP MF_00186		PRO_1000098710
Regions
	Nucleotide binding	408 – 412	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	264	1	ATP By similarity
	Binding site	307	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A923-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 33999EDC9AF9CD0C	FASTA	495	53,124
	10 20 30 40 50 60 MQAILALDQG TTSSRAIVYD AHGGVLGTAQ QDFPQYFPQP GWVEHDPGEI WQSQYRVMIQ 70 80 90 100 110 120 AVERAGIPWS AIAGLGLTNQ RETTLLWDRA TGEPLHRAIV WQDRRTARLC DDLRRDGHER 130 140 150 160 170 180 LFRERTGLLL DPYFSGTKLR WLLDHVPGAR RRAEAGELAF GTVDSWLIWQ LTGGRLHLTD 190 200 210 220 230 240 ASNASRTLLC NIHSGDWDPD LLAALDIPAA LLPEIIDSSG VCGTTCCAGV PEGIPIAGVA 250 260 270 280 290 300 GDQQAALYGQ GCHEAGLAKC TYGTGAFLLM HTGERPIASA NRLLTTVAWR IGGRTAYALE 310 320 330 340 350 360 GSVFIAGAVV QWLRDGLGLI RSSDEIEALA RQVPDTGGVY LVPAFAGLGA PHWDPDARGI 370 380 390 400 410 420 LVGMTRGTER PHIARAALES MAFQATEVIG AMEVDAGLAV KELRVDGGAS ANDLLMQFQA 430 440 450 460 470 480 DLLGAPVLRP ADTEATAAGA AALAARAVGL NGARPSAEAA FTAFSPRLSR YEVEQRMATW 490 QRAVRRAGGW ARDDD

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI56664.1.
RefSeq	YP_742154.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A923.
Genome annotation databases
GeneID	4268654.
GenomeReviews	Gene locus Mlg_1315 in contig CP000453_GR.
KEGG	aeh:Mlg_1315.
NMPDR	fig|187272.6.peg.1261.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0A923.
OMA	SIDERND.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ALHEH

Accession

Primary (citable) accession number: Q0A923

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)