Reviewed, UniProtKB/Swiss-Prot Q0A8V7 (DXS_ALHEH)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Mlg_1381

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019003

Sequences

	Sequence		Length	Mass (Da)
	Q0A8V7-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 484808410EB0611E	FASTA	628	67,680
	10 20 30 40 50 60 MSVSIDDYPL LAQVPDPEAL RTLPAHRLPA LAKELRHYLL HSVARSGGHL AAGLGAVELT 70 80 90 100 110 120 LALHYVFNTP EDRLVWDVGH QCYPHKILTG RRERLGTIRK YGGLAGFPKR AESPYDTFGV 130 140 150 160 170 180 GHSSTSISAA LGMALAARQA GEQRKAVAII GDGGMTAGEA FEAMNHAGDA GADLLVVLND 190 200 210 220 230 240 NEMSISENVG ALSQHLTRIL SGRWYHQLRS GSKEVLRRLP PPVHELARRT EEHLKGMVVP 250 260 270 280 290 300 GTLFEELGFQ YFGPVDGHNV DALVEVLGNL AHQRGPRLLH VVTCKGKGYR PAEQDPIAYH 310 320 330 340 350 360 GVGQFDPEQG LPKKSGGSLA YNQVFGRWLC AMAEQDPRLV AITPAMREGS GMVEYARRFP 370 380 390 400 410 420 ERYHDVGIAE QHAVTLAAGL ACESVKPVLA IYSTFLQRGY DQLVHDVALQ NLPVLFAVDR 430 440 450 460 470 480 AGLVGADGPT HHGSFDLSYL RCVPNMTIAA PSDEAECWRL LSTGYHHDGP FAVRYPRGSG 490 500 510 520 530 540 PGAALPEADL DPLAIGKGVC RRRGRRIAVL AFGTLVVPAL AVAEALDLTV ADMRFVRPLD 550 560 570 580 590 600 EALIRELADT HDLLVTVEEN AVAGGAGSGV SEYLARAGLD VPVRHLGLPD RFVDHGTPAE 610 620 LLAEVGLDEA GLQRSLQGWL DTLPGASR

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI56730.1.
RefSeq	YP_742220.1.
3D structure databases
SMR	Q0A8V7. Positions 4-620.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A8V7.
Genome annotation databases
GeneID	4269573.
GenomeReviews	Gene locus Mlg_1381 in contig CP000453_GR.
KEGG	aeh:Mlg_1381.
NMPDR	fig|187272.6.peg.1326.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	LLNDNEM.
PhylomeDB	Q0A8V7.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ALHEH

Accession

Primary (citable) accession number: Q0A8V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)