Reviewed, UniProtKB/Swiss-Prot Q0A8K7 (SYA_ALHEH)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Mlg_1481

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	869 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 869	869	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347484

Sequences

	Sequence		Length	Mass (Da)
	Q0A8K7-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 033C4BAC7D8AA86B	FASTA	869	95,318
	10 20 30 40 50 60 MKSSAEIRNA FLEFFEHRGH TVVPSSPLVP ANDPTLLFTN AGMVPFKDVF LGKEQRGYTR 70 80 90 100 110 120 ACSSQRCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKREAIEYA WEFLTRTIGL 130 140 150 160 170 180 PAERLWVTVY EEDDEAADIW LNEIGVDPER FGRIGAADNF WSMGDTGPCG PCSEIFYDHG 190 200 210 220 230 240 PEVPGGPPGS PEEDGDRYVE IWNLVFMQYD RDAEGRLAPL PMPCVDTGMG LERLAAVVQG 250 260 270 280 290 300 VHSNFEIDLF RRLIGAAAAL AGLPEDSDNA SLKVIADHIR ACAFLITDGV VPANDGRGYV 310 320 330 340 350 360 LRRIIRRAVR HGYKLGIDEP FFHRLVQPLA DEMGGAFPEL PERQALVERL LLQEEQRFRE 370 380 390 400 410 420 TLEQGLKLLE EDLRQLTGAE IPGETVFKLA DTYGFPVDLT ADIARERDLT LDMAGFEACM 430 440 450 460 470 480 AAQRERARAH SQFKVQHGEG EQFEGESHFI GYDQLEGHGE VLALFRDGRS VQTLSAGEEG 490 500 510 520 530 540 MVILDQTPFY AESGGQVGDQ GVLETAGGEV FEVRDTLKQG EGHGHLGRLR EGRIHVGDRL 550 560 570 580 590 600 RAQVDAETRW ATALNHSATH LLHAALRGVL GTHVQQKGSL VAPDRLRFDF AHYEAPSTEQ 610 620 630 640 650 660 LEQIERIVND EIRANRAADI EHMAYDDAIE TGAMALFGEK YGDQVRVLRF GEFSIELCGG 670 680 690 700 710 720 THVERTGDIG LCKLISEGGV AGGVRRIEAV TGDRAVMWVQ RTEQRLVQVA ETVKASPDNA 730 740 750 760 770 780 AVRVAQLVDR LKGQEKELER LKQKLASQAG SDLAGQAVDI GGVKVVAARV DADNKALRDT 790 800 810 820 830 840 VDQLRNKLGT AVIVLGAVAG DKVRLVAGVS KDCTDRIKAG DLVRQVAEQV GGKGGGRPDF 850 860 AQAGGEQPEH LETALASVES LVRDALGLE

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI56830.1.
RefSeq	YP_742320.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A8K7.
Genome annotation databases
GeneID	4269954.
GenomeReviews	Gene locus Mlg_1481 in contig CP000453_GR.
KEGG	aeh:Mlg_1481.
NMPDR	fig|187272.6.peg.1421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0A8K7.
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ALHEH

Accession

Primary (citable) accession number: Q0A8K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)