Q0A8G6 (KATG_ALHEH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Mlg_1522

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Catalase-peroxidase HAMAP MF_01961		PRO_0000354717
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A8G6 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: FFE8AFD8FC97D3DE	FASTA	723	80,878
	10 20 30 40 50 60 MGDQQNAGKC PVMHGAMTSA GKSNMDWWPN NLNLDILHQH DRKTNPMGED FDYREEVQKL 70 80 90 100 110 120 DFDAVKKDLT ELMTNSQDWW PADWGHYGGL MIRMAWHAAG SYRVADGRGG GGTGNQRFAP 130 140 150 160 170 180 INSWPDNANL DKARRLLWPI KKKYGNKLSW ADLIILAGNV AYESMGFKTF GFSFGREDIW 190 200 210 220 230 240 HPEKDTYWGS EKEWLAPSDN PESRYSGDRE SLENPLAAVM MGLIYVNPEG VDGNPDPLKT 250 260 270 280 290 300 AEDVRVTFAR MAMNDEETVA LTAGGHTVGK CHGNGDAEKI GPEPEAADVE EQGLGWRNLA 310 320 330 340 350 360 SRGVGRDTVT SGLEGAWTTH PTRWDDGYFD MLLNHEWELT KSPAGAWQWQ PVDIKEEDMP 370 380 390 400 410 420 VDVEDPSIRH MPMMTDADMA MKMDPEYRKY AERFQKDPEY FNEVFARAWF KLIHRDMGPK 430 440 450 460 470 480 TRYIGPEAPD EDLIWQDPVP AGNTGYDVDA VKAKIADSGL SISEMVATAW DSARTFRGSD 490 500 510 520 530 540 YRGGANGARI RLAPQKDWEG NEPERLSKVL GVLEGIAADT GASVADTIVL AGNVGIEQAA 550 560 570 580 590 600 KAAGHDITVP FAPGRGDASQ EMTDEESFEY LEPLSDGYRN WLKKDYAVAP EEMMLDRTQL 610 620 630 640 650 660 LGLTAPEMTV LIGGMRVLGT NHGGTQHGVF TDREGQLTND FFVNLTDMNY AWEPVGNNLY 670 680 690 700 710 720 EIRDRKTGET RWTATRVDLV FGSNSILRSY AEVYAQDDNK EKFVKDFVAA WNKVMNADRF DLA

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MLHE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI56871.1.
RefSeq	YP_742361.1. NC_008340.1.
3D structure databases
ProteinModelPortal	Q0A8G6.
SMR	Q0A8G6. Positions 23-722.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A8G6.
Protein family/group databases
PeroxiBase	3628. AeCP01_MLHE-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4269078.
GenomeReviews	Gene locus Mlg_1522 in contig CP000453_GR.
KEGG	aeh:Mlg_1522.
NMPDR	fig|187272.6.peg.1462.
PATRIC	20862301. VBIAlkEhr114327_1524.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q0A8G6.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	AEHR187272:MLG_1522-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ALHEH

Accession

Primary (citable) accession number: Q0A8G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program