Q0A845 (MASZ_ALHEH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Mlg_1646

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola › Alkalilimnicola ehrlichii

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Malate synthase G HAMAP-Rule MF_00641		PRO_1000130886
Sites
	Active site	344	1	Proton acceptor By similarity
	Active site	635	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A845 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 05A52DB21B7F6187	FASTA	729	80,444
	10 20 30 40 50 60 MTQRIEVGGL QVAQELYDLV AKEIAPATGV DADGFWTHLG AIVRDLGARN RELLQKRDEI 70 80 90 100 110 120 QQAIDKWHLE RKGQPHDAAA YKTFLEELGY LQPEGEDFEA TTENVDPEIA EVAGPQLVVP 130 140 150 160 170 180 INNARFALNA ANARWGSLYD ALYGSDVLPE DDGAEKGTRY NPVRGKKVME YAGRVLDEVA 190 200 210 220 230 240 PLAQGRHGDV VAYNLVDDNG RKRLQARLSD GSETGLADPE GLAGYNGAED DPTVLLLRHN 250 260 270 280 290 300 GLHVEIQIDR EHLIGKDHPA GVKDVVMEAA VTAIMDCEDS VAAVDAEDKT AVYRNWLGLM 310 320 330 340 350 360 NGDLETSLEK GGKTIQRRLN ADRDYIGADG RALTLPGRSL MLIRNVGHLM TTPAVLDADG 370 380 390 400 410 420 NEIPEGILDG MVTSLIALYD LKGLGRYRNS RSGSMYIVKP KMHGPDEVAF AVELFGRIED 430 440 450 460 470 480 ALGMERNTLK IGVMDEERRT TVNLKACIRE AKERIIFINT GFLDRTGDEM HTSMEAGPMI 490 500 510 520 530 540 RKAEMKSSRW LNAYEDWNVD IGLECGLRGR AQIGKGMWTM PDLMKGMMEA KIGHPKAGAN 550 560 570 580 590 600 CAWVPSPTAA TLHAIHYHQV NVAEVQDRLA GQRRATLDDL LTIPLEPSPS WSAEEIQQEL 610 620 630 640 650 660 DNNCQGILGY VVRWVDQGIG CSKVPDINNV GLMEDRATLR IASQLLANWL HHGICSEEQI 670 680 690 700 710 720 METMKRMAKV VDQQNAGDPN YRPMAADYDG SVAFQAACEL VLKGREQPSG YTEPVLHRRR REAKAKFGA

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MLHE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI56992.1.
RefSeq	YP_742482.1. NC_008340.1.
3D structure databases
ProteinModelPortal	Q0A845.
SMR	Q0A845. Positions 2-726.
ModBase	Search...
Protein-protein interaction databases
STRING	187272.Mlg_1646.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI56992; ABI56992; Mlg_1646.
GeneID	4270247.
KEGG	aeh:Mlg_1646.
PATRIC	20862557. VBIAlkEhr114327_1649.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	VVKPKMH.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	AEHR187272:GHAX-1702-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_ALHEH

Accession

Primary (citable) accession number: Q0A845

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program