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Protein

Malate synthase G

Gene

glcB

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei280Acetyl-CoAUniRule annotation1
Binding sitei317Acetyl-CoAUniRule annotation1
Active sitei344Proton acceptorUniRule annotation1
Binding sitei344GlyoxylateUniRule annotation1
Metal bindingi436MagnesiumUniRule annotation1
Binding sitei436GlyoxylateUniRule annotation1
Metal bindingi464MagnesiumUniRule annotation1
Binding sitei545Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei635Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Mlg_1646
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
Proteomesi
  • UP000001962 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001308861 – 729Malate synthase GAdd BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei621Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187272.Mlg_1646.

Structurei

3D structure databases

ProteinModelPortaliQ0A845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni461 – 464Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0A845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQRIEVGGL QVAQELYDLV AKEIAPATGV DADGFWTHLG AIVRDLGARN
60 70 80 90 100
RELLQKRDEI QQAIDKWHLE RKGQPHDAAA YKTFLEELGY LQPEGEDFEA
110 120 130 140 150
TTENVDPEIA EVAGPQLVVP INNARFALNA ANARWGSLYD ALYGSDVLPE
160 170 180 190 200
DDGAEKGTRY NPVRGKKVME YAGRVLDEVA PLAQGRHGDV VAYNLVDDNG
210 220 230 240 250
RKRLQARLSD GSETGLADPE GLAGYNGAED DPTVLLLRHN GLHVEIQIDR
260 270 280 290 300
EHLIGKDHPA GVKDVVMEAA VTAIMDCEDS VAAVDAEDKT AVYRNWLGLM
310 320 330 340 350
NGDLETSLEK GGKTIQRRLN ADRDYIGADG RALTLPGRSL MLIRNVGHLM
360 370 380 390 400
TTPAVLDADG NEIPEGILDG MVTSLIALYD LKGLGRYRNS RSGSMYIVKP
410 420 430 440 450
KMHGPDEVAF AVELFGRIED ALGMERNTLK IGVMDEERRT TVNLKACIRE
460 470 480 490 500
AKERIIFINT GFLDRTGDEM HTSMEAGPMI RKAEMKSSRW LNAYEDWNVD
510 520 530 540 550
IGLECGLRGR AQIGKGMWTM PDLMKGMMEA KIGHPKAGAN CAWVPSPTAA
560 570 580 590 600
TLHAIHYHQV NVAEVQDRLA GQRRATLDDL LTIPLEPSPS WSAEEIQQEL
610 620 630 640 650
DNNCQGILGY VVRWVDQGIG CSKVPDINNV GLMEDRATLR IASQLLANWL
660 670 680 690 700
HHGICSEEQI METMKRMAKV VDQQNAGDPN YRPMAADYDG SVAFQAACEL
710 720
VLKGREQPSG YTEPVLHRRR REAKAKFGA
Length:729
Mass (Da):80,444
Last modified:October 17, 2006 - v1
Checksum:i05A52DB21B7F6187
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI56992.1.
RefSeqiWP_011629386.1. NC_008340.1.

Genome annotation databases

EnsemblBacteriaiABI56992; ABI56992; Mlg_1646.
KEGGiaeh:Mlg_1646.
PATRICi20862557. VBIAlkEhr114327_1649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA. Translation: ABI56992.1.
RefSeqiWP_011629386.1. NC_008340.1.

3D structure databases

ProteinModelPortaliQ0A845.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187272.Mlg_1646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI56992; ABI56992; Mlg_1646.
KEGGiaeh:Mlg_1646.
PATRICi20862557. VBIAlkEhr114327_1649.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_ALKEH
AccessioniPrimary (citable) accession number: Q0A845
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.