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Q0A812 (CYSG_ALKEH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Siroheme synthase

Including the following 3 domains:

  1. Uroporphyrinogen-III C-methyltransferase
    Short name=Urogen III methylase
    EC=2.1.1.107
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name=UROM
  2. Precorrin-2 dehydrogenase
    EC=1.3.1.76
  3. Sirohydrochlorin ferrochelatase
    EC=4.99.1.4
Gene names
Name:cysG
Ordered Locus Names:Mlg_1679
OrganismAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) [Complete proteome] [HAMAP]
Taxonomic identifier187272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity. HAMAP-Rule MF_01646

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP-Rule MF_01646

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP-Rule MF_01646

Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP-Rule MF_01646

Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP-Rule MF_01646

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.

In the C-terminal section; belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481Siroheme synthase HAMAP-Rule MF_01646
PRO_0000330492

Regions

Nucleotide binding22 – 232NAD By similarity
Nucleotide binding43 – 442NAD By similarity
Region1 – 202202precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Region218 – 481264Uroporphyrinogen-III C-methyltransferase By similarity
Region303 – 3053S-adenosyl-L-methionine binding By similarity
Region333 – 3342S-adenosyl-L-methionine binding By similarity

Sites

Active site2501Proton acceptor By similarity
Active site2721Proton donor By similarity
Binding site2271S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site3081S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site3851S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site4141S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0A812 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 3F06CE71D23F8CE9

FASTA48152,927
        10         20         30         40         50         60 
METLPVFMKL RDRRCLVVGG GRRAERKARL LLAAGAELTV LAEAPLPTLA ALTEAHGCRM 

        70         80         90        100        110        120 
VRRPLTARDL DGVSLVISAA DEATDRRAHM LARARNIPIN VVDRPDLCSF TLPATVDRGP 

       130        140        150        160        170        180 
VQIAVSTGGT SPVLARMLRN RLEADIPSAY GRLARLAERY RRPVREVLPE AWQRQRFWEE 

       190        200        210        220        230        240 
VLSGEVAERV FAGQDDAARE GLEEAIGRAT RELQTRRGEV YLVGAGPGDP DLLTLRALRL 

       250        260        270        280        290        300 
MQQADAVVYD RLVNPAIMAK VNQDAERIDV GKRCGHHPVP QHAINDKLVT LARQGYRVLR 

       310        320        330        340        350        360 
LKGGDPFVFG RGGEELQTLV DAGVPFQVVP GITAATGCAA YSGIPLTHRD YAHSCAFYTG 

       370        380        390        400        410        420 
HLKNDRLDLD WSRMVQPGQT LVFYMGVASL PELSRQLCWH GLPAETPAAL VEKGTTPEQR 

       430        440        450        460        470        480 
TLTATLETLP GLARQQGFQS PALVIIGEVV RLYEQINWYR PPGDETPKAA EPGELNRRRP 


A 

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References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000453 Genomic DNA. Translation: ABI57025.1.
RefSeqYP_742515.1. NC_008340.1.

3D structure databases

ProteinModelPortalQ0A812.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187272.Mlg_1679.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI57025; ABI57025; Mlg_1679.
GeneID4268911.
KEGGaeh:Mlg_1679.
PATRIC20862627. VBIAlkEhr114327_1684.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0007.
HOGENOMHOG000290518.
KOK02302.
OrthoDBEOG6DRPFR.

Enzyme and pathway databases

BioCycAEHR187272:GHAX-1723-MONOMER.
UniPathwayUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Family and domain databases

Gene3D1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_01646. Siroheme_synth.
InterProIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR028281. Sirohaem_synthase_central.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMSSF53790. SSF53790. 1 hit.
TIGRFAMsTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSG_ALKEH
AccessionPrimary (citable) accession number: Q0A812
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 17, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways