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Q0A7N2

- SYE2_ALKEH

UniProt

Q0A7N2 - SYE2_ALKEH

Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi109 – 1091ZincUniRule annotation
    Metal bindingi111 – 1111ZincUniRule annotation
    Metal bindingi136 – 1361ZincUniRule annotation
    Metal bindingi138 – 1381ZincUniRule annotation
    Binding sitei256 – 2561ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAEHR187272:GHAX-1855-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetase 2UniRule annotation
    Short name:
    GluRS 2UniRule annotation
    Gene namesi
    Name:gltX2UniRule annotation
    Ordered Locus Names:Mlg_1811
    OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
    Taxonomic identifieri187272 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
    ProteomesiUP000001962: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Glutamate--tRNA ligase 2PRO_0000367606Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi187272.Mlg_1811.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0A7N2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2211"HIGH" regionAdd
    BLAST
    Motifi253 – 2575"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252721.
    KOiK01885.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0A7N2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPTNVKTRF APSPTGRMHL GNLRTALFNA LLARSRGGRF LLRLEDTDES    50
    RSSGAHAQTL MADLRWMGLH WDEGPEAGGE AGPYHQSERG AVYERYYRAL 100
    EAADQAYPCF CTERELELSR KAQRAAGKPP RYAGTCAHLT AEERERRRAE 150
    GRRPTLRFRV PAEETVVFHD LVRGEQRFPT DEIGDFIIRR ADGTAAFFFC 200
    NAVDDALMGV SHVLRGEDHL TNTPRQLLLL RALDLPQPEY GHINLITGDD 250
    GAPLSKRNGS LSVAELREAG WLPEAVLNYL ARLGHHYTGE VESQLLDLQG 300
    LADAFRTDAL GRAPARFDRH QLSHWQQLAV HRADEATLAP WLERLDDAVP 350
    ADRRRALLAA VRDNILFPDD LAAWGQRVFG PLPALAPDTE AVIRDAGPAF 400
    YEAALAELET TAGDFPALAK AVRKATGAKG RQLFMPLRAA LTGLCRGPEL 450
    GPVYALMPVD IARCRLEQAR ELAATTP 477
    Length:477
    Mass (Da):52,785
    Last modified:October 17, 2006 - v1
    Checksum:i679DCC6139DDF3A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000453 Genomic DNA. Translation: ABI57155.1.
    RefSeqiYP_742645.1. NC_008340.1.

    Genome annotation databases

    EnsemblBacteriaiABI57155; ABI57155; Mlg_1811.
    GeneIDi4269473.
    KEGGiaeh:Mlg_1811.
    PATRICi20862879. VBIAlkEhr114327_1810.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000453 Genomic DNA. Translation: ABI57155.1 .
    RefSeqi YP_742645.1. NC_008340.1.

    3D structure databases

    ProteinModelPortali Q0A7N2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187272.Mlg_1811.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI57155 ; ABI57155 ; Mlg_1811 .
    GeneIDi 4269473.
    KEGGi aeh:Mlg_1811.
    PATRICi 20862879. VBIAlkEhr114327_1810.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252721.
    KOi K01885.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci AEHR187272:GHAX-1855-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1101 / DSM 17681 / MLHE-1.

    Entry informationi

    Entry nameiSYE2_ALKEH
    AccessioniPrimary (citable) accession number: Q0A7N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3