Q0A7L5 (PIMT_ALHEH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	Mlg_1828

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola › Alkalilimnicola ehrlichii

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Protein-L-isoaspartate O-methyltransferase HAMAP-Rule MF_00090		PRO_0000351811
Sites
	Active site	70	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A7L5 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: E828CC9C2CBAC15A	FASTA	221	24,679
	10 20 30 40 50 60 MDPNRYQGIG MTSRRTRERL VSRLAEEGIR DPRVLQAILE VPRHLFVDEA LASRAYDNTP 70 80 90 100 110 120 LPIGHGQTIS QPWVVARMTE LLIEQSIPER VLELGTGSGY QAAVLAYLGV EVYTIERIKA 130 140 150 160 170 180 LADQARQRMR DLRLHRVHVR YGDGSEGWAQ HAPYQGIIVT AAPEEVPDPL WDQLDEGGRL 190 200 210 220 VAPLGGAGRP QELVLIERVD GELRRRHVAS VSFVPLLGGC R

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MLHE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI57172.1.
RefSeq	YP_742662.1. NC_008340.1.
3D structure databases
ProteinModelPortal	Q0A7L5.
ModBase	Search...
Protein-protein interaction databases
STRING	187272.Mlg_1828.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI57172; ABI57172; Mlg_1828.
GeneID	4268183.
KEGG	aeh:Mlg_1828.
PATRIC	20862913. VBIAlkEhr114327_1827.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	VRARMVQ.
ProtClustDB	CLSK727475.
Enzyme and pathway databases
BioCyc	AEHR187272:GHAX-1884-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_ALHEH

Accession

Primary (citable) accession number: Q0A7L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program