Reviewed, UniProtKB/Swiss-Prot Q0A7K2 (PYRG_ALHEH)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	Mlg_1841

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	CTP synthase HAMAP MF_01227		PRO_0000266051
Regions
	Domain	290 – 540	251	Glutamine amidotransferase type-1
	Region	1 – 252	252	Aminator domain HAMAP MF_01227
Sites
	Active site	378	1	Nucleophile By similarity
	Active site	514	1	By similarity
	Active site	516	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A7K2-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 7F703139C3127293	FASTA	540	60,166
	10 20 30 40 50 60 MTRYIFITGG VVSSLGKGIT AASLGTILQA RGLSVSMTKL DPYINVDPGT MSPFQHGEVY 70 80 90 100 110 120 VTDDGAETDL DLGHYERFVR TTMTRNNNYT TGRIYESVIR KERRGEYLGG TVQVIPHITD 130 140 150 160 170 180 EIKRSIQQGA DDADIALIEI GGTVGDIESL PFLEAIRQMG AELGRGRCLF MHLTLVPFIG 190 200 210 220 230 240 AAGEMKTKPT QHSVKELRSI GIQPDILVCR ASQRIPEEER RKIALFTNVE PRAVVSCLDV 250 260 270 280 290 300 DNIYKIPEVL HRQGLDNIVA EKLGLELPPA SLQDWQRVVE AMQNPEGEVT IAMVGKYVDL 310 320 330 340 350 360 TDAYMSLNES LRHAGIQTRH RVNIRYIDSE ELEREGTHAL DGVDAVLVPG GFGERGVEGK 370 380 390 400 410 420 ILAARYARER KVPYLGICLG MQVAVIEYAR NVAGLEGAHS TEFTRHPHHP VIGLITEWMT 430 440 450 460 470 480 DEGTVEQRSE DSDLGGTMRL GAQPCRLTEG SLARQVYGKD VVEERHRHRY EFNNHYLEAL 490 500 510 520 530 540 EAAGLRFSGW SHDRKLVEVV EQPDHPWFLA CQFHPEFTST PRDGHPLFAA FVRAAIAHRG

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI57185.1.
RefSeq	YP_742675.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4269209.
GenomeReviews	Gene locus Mlg_1841 in contig CP000453_GR.
KEGG	aeh:Mlg_1841.
NMPDR	fig|187272.6.peg.1752.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0A7K2.
OMA	Q0A7K2. EFNNAYR.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_ALHEH

Accession

Primary (citable) accession number: Q0A7K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)