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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi488MagnesiumUniRule annotation1
Metal bindingi494MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciAEHR187272:G1G7A-1992-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Mlg_1944
OrganismiAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)
Taxonomic identifieri187272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003294901 – 698Polyribonucleotide nucleotidyltransferaseAdd BLAST698

Proteomic databases

PRIDEiQ0A7A1

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi187272.Mlg_1944

Structurei

3D structure databases

ProteinModelPortaliQ0A7A1
SMRiQ0A7A1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini555 – 614KHUniRule annotationAdd BLAST60
Domaini624 – 692S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218326
KOiK00962
OrthoDBiPOG091H00M0

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 2 hits
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q0A7A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVKKSFQY GNHTVTLETG GVARQADGAV LVNMSDTVVL VTAVGRKEAD
60 70 80 90 100
PGKGFFPLTV NYQERTYAAG KIPGGFFKRE GRPSEKETLT CRLIDRPIRP
110 120 130 140 150
LFPEGFYNEV QVVATVLSMN PEVDADIPAL IGASAALSIS GIPFDGPIGA
160 170 180 190 200
ARVGYKDGEY LLNPTFEETA ASDLDLVVAG TENAVLMVES EANQLPEEAM
210 220 230 240 250
LGAVLYGHEQ MQVAIQAINE LTAEAGKPRW DWHPPQGDAA LETAIKDLVG
260 270 280 290 300
DDLAAAYQIP EKQERQNRIG ELRQRAVEAL GENREEEGGW PEKDVGDAFK
310 320 330 340 350
GLEKDIVRGR ILAGERRIDG RDTRTVRPID IEVGSLPRTH GSAIFTRGET
360 370 380 390 400
QAVVVTTLGT GRDAQIIDAI EGERKEQFML HYNFPPYCVG ETGFMGTPKR
410 420 430 440 450
REIGHGKLAK RGIEAVMPAA DDCPYVIRVV SEITESNGSS SMATVCGTSL
460 470 480 490 500
SLMDAGVPVK APVAGIAMGL IKEDEQFAVL SDILGDEDHL GDMDFKVAGT
510 520 530 540 550
ESGVTALQMD IKIQGITREI MEQALEQARE GRLHILGEMN NAISGPRSEM
560 570 580 590 600
SEYAPRLLTI RIDPDKIRDV IGKGGATIRA LTEETGTTID ISDDGKVTIA
610 620 630 640 650
SADKAAADEA RRRIELLTAD VEVGTVYEGK VSKLMDFGAF VNILPGRDGL
660 670 680 690
VHISQISNER VERVGDYLKE GDTVRVKVLE VDRQGRIRLS MKAVQDGE
Length:698
Mass (Da):75,642
Last modified:April 29, 2008 - v2
Checksum:iE0A4CEEC4D99642F
GO

Sequence cautioni

The sequence ABI57286 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000453 Genomic DNA Translation: ABI57286.1 Different initiation.
RefSeqiWP_041718014.1, NC_008340.1

Genome annotation databases

EnsemblBacteriaiABI57286; ABI57286; Mlg_1944
KEGGiaeh:Mlg_1944

Similar proteinsi

Entry informationi

Entry nameiPNP_ALKEH
AccessioniPrimary (citable) accession number: Q0A7A1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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