Reviewed, UniProtKB/Swiss-Prot Q0A7A1 (PNP_ALHEH)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	Mlg_1944

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 698	698	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329490
Regions
	Domain	555 – 614	60	KH
	Domain	624 – 692	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q0A7A1-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: E0A4CEEC4D99642F	FASTA	698	75,642
	10 20 30 40 50 60 MKSVKKSFQY GNHTVTLETG GVARQADGAV LVNMSDTVVL VTAVGRKEAD PGKGFFPLTV 70 80 90 100 110 120 NYQERTYAAG KIPGGFFKRE GRPSEKETLT CRLIDRPIRP LFPEGFYNEV QVVATVLSMN 130 140 150 160 170 180 PEVDADIPAL IGASAALSIS GIPFDGPIGA ARVGYKDGEY LLNPTFEETA ASDLDLVVAG 190 200 210 220 230 240 TENAVLMVES EANQLPEEAM LGAVLYGHEQ MQVAIQAINE LTAEAGKPRW DWHPPQGDAA 250 260 270 280 290 300 LETAIKDLVG DDLAAAYQIP EKQERQNRIG ELRQRAVEAL GENREEEGGW PEKDVGDAFK 310 320 330 340 350 360 GLEKDIVRGR ILAGERRIDG RDTRTVRPID IEVGSLPRTH GSAIFTRGET QAVVVTTLGT 370 380 390 400 410 420 GRDAQIIDAI EGERKEQFML HYNFPPYCVG ETGFMGTPKR REIGHGKLAK RGIEAVMPAA 430 440 450 460 470 480 DDCPYVIRVV SEITESNGSS SMATVCGTSL SLMDAGVPVK APVAGIAMGL IKEDEQFAVL 490 500 510 520 530 540 SDILGDEDHL GDMDFKVAGT ESGVTALQMD IKIQGITREI MEQALEQARE GRLHILGEMN 550 560 570 580 590 600 NAISGPRSEM SEYAPRLLTI RIDPDKIRDV IGKGGATIRA LTEETGTTID ISDDGKVTIA 610 620 630 640 650 660 SADKAAADEA RRRIELLTAD VEVGTVYEGK VSKLMDFGAF VNILPGRDGL VHISQISNER 670 680 690 VERVGDYLKE GDTVRVKVLE VDRQGRIRLS MKAVQDGE

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000453 Genomic DNA. Translation: ABI57286.1. Different initiation.
RefSeq	YP_742776.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4268112.
GenomeReviews	Gene locus Mlg_1944 in contig CP000453_GR.
KEGG	aeh:Mlg_1944.
NMPDR	fig|187272.6.peg.1852.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0A7A1.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_ALHEH

Accession

Primary (citable) accession number: Q0A7A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	June 16, 2009
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)