Reviewed, UniProtKB/Swiss-Prot Q0A6U0 (METE_ALHEH)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	Mlg_2105

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017218
Sites
	Metal binding	647	1	Zinc By similarity
	Metal binding	649	1	Zinc By similarity
	Metal binding	732	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A6U0-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: E1F7BED8F33CD015	FASTA	773	86,615
	10 20 30 40 50 60 MSTTHVLGYP RIGARRELKR ATEAYWKGEI DQAELERTGR ELRARHWQAQ RDAGLDWVTV 70 80 90 100 110 120 GDFAFYDHVL NVSALLGAVP PRFGDAGETV DLDTTFRMAR GRAPTGEPAA ACEMTKYFDT 130 140 150 160 170 180 NYHYLVPELH ADQRFGLASR RLFDEVAEAR AAGHPVKVVL TGPLTWLWLG KAKSPGLDRL 190 200 210 220 230 240 DLLDGVVDVY GEILQQLGEQ GVEWVQLDEP ALVQDLPADW QQAYERAYHR LQAGPVKLLL 250 260 270 280 290 300 ATYFGGLGEN LSTALNLPVD GIHIDCVRDP DQLTAVLDRL LPHKVLSLGV IDGRNVWRSD 310 320 330 340 350 360 LAALARTLAP VRERLGERLW LAPSCSLLHV PVDLDLETEL QPQLRRWLAF ARQKLDELVV 370 380 390 400 410 420 LGRLLGDRAG AADEQAAAEA SAALADRQAS THIHKAEVAE RMQRVGPADR QRGQPYPERA 430 440 450 460 470 480 RKQRKRLALP LFPTTTIGSF PQTAEIRAAR RDLKAGRLTP EAYEARMREE IAFAVARQEE 490 500 510 520 530 540 IGLDVLVHGE AERNDMVEYF GEQLDGFAFT RAGWVQSYGS RCVKPPIIYG DVSRPRPMTV 550 560 570 580 590 600 RWSEYAQSLT GRPMKGMLTG PVTVLQWSFV RDDQPRSETC RQIALALRDE VADLEAAGIP 610 620 630 640 650 660 AIQIDEPALR EGLPLQQRQW PGYLDWAVDC FRLTASVAQD ATQIHTHMCY SEFNDIMGAI 670 680 690 700 710 720 ATLDADVITI ETSRSDMELL EVFEGFEYPN EIGPGVYDIH SPNTPSVDWM VRLMEKAARR 730 740 750 760 770 IPVERLWVNP DCGLKTRAWA EVEPALQNMV AAAEILRERY GQAQTRVATQ GKR

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI57447.1.
RefSeq	YP_742937.1.
3D structure databases
SMR	Q0A6U0. Positions 3-759.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A6U0.
Genome annotation databases
GeneID	4270083.
GenomeReviews	Gene locus Mlg_2105 in contig CP000453_GR.
KEGG	aeh:Mlg_2105.
NMPDR	fig|187272.6.peg.2005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	SFVRNDI.
PhylomeDB	Q0A6U0.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_ALHEH

Accession

Primary (citable) accession number: Q0A6U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)