ID   DDL_ALHEH               Reviewed;         311 AA.
AC   Q0A6K5;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 28.
DE   RecName: Full=D-alanine--D-alanine ligase;
DE            EC=6.3.2.4;
DE   AltName: Full=D-alanylalanine synthetase;
DE   AltName: Full=D-Ala-D-Ala ligase;
GN   Name=ddl; OrderedLocusNames=Mlg_2190;
OS   Alkalilimnicola ehrlichei (strain MLHE-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Alkalilimnicola.
OX   NCBI_TaxID=187272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T.,
RA   King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T.,
RA   Xie G., Stolz J.F., Richardson P.;
RT   "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cell wall formation (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC       alanyl-D-alanine.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; CP000453; ABI57532.1; -; Genomic_DNA.
DR   RefSeq; YP_743022.1; -.
DR   GeneID; 4270969; -.
DR   GenomeReviews; CP000453_GR; Mlg_2190.
DR   KEGG; aeh:Mlg_2190; -.
DR   NMPDR; fig|187272.6.peg.2087; -.
DR   HOGENOM; Q0A6K5; -.
DR   OMA; Q0A6K5; VPYTGSD.
DR   GO; GO:0005618; C:cell wall; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007047; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_00047; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR000291; D-Ala_lig_Van_CS.
DR   InterPro; IPR005905; D_ala_D_ala.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR011127; Dala_Dala_lig_N.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF07478; Dala_Dala_lig_C; 1.
DR   Pfam; PF01820; Dala_Dala_lig_N; 1.
DR   TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR   PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW   Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT   CHAIN         1    311       D-alanine--D-alanine ligase.
FT                                /FTId=PRO_0000341049.
FT   DOMAIN      106    301       ATP-grasp.
FT   NP_BIND     132    187       ATP (By similarity).
FT   METAL       255    255       Magnesium or manganese 1 (By similarity).
FT   METAL       268    268       Magnesium or manganese 1 (By similarity).
FT   METAL       268    268       Magnesium or manganese 2 (By similarity).
FT   METAL       270    270       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   311 AA;  33873 MW;  BCFD7FC34464A85B CRC64;
     MSSEQAAAMG RVAVLMGGDS AEREISLISG RAVFEALRRR GVDAEAVDTA QVALAELTGF
     DRAFIALHGR GGEDGVIQGA LEALGVPYTG SGVLGCAIGM DKWRTKLLWR GAELPVPPGA
     LLRPETNRQA LIGSVGLPLM IKPAHEGSSI GMAKVERPEE LEAARAEAAR YDDLVLAERW
     IEGGEYTVAI LGEEALPAIR LETPRGFYDF EAKYRSGDTR YHCPAGLTEA EEQQIRQLAL
     DAFRVAGASG WGRVDFMRDR QGRFWLLEIN TIPGMTDHSL VPMAARAVGI DFDELCWRIL
     LDSRRREAGR P
//