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Q0A6K5 (DDL_ALKEH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Mlg_2190
OrganismAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) [Complete proteome] [HAMAP]
Taxonomic identifier187272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341049

Regions

Domain106 – 301196ATP-grasp
Nucleotide binding132 – 18756ATP By similarity

Sites

Metal binding2551Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 2 By similarity
Metal binding2701Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0A6K5 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: BCFD7FC34464A85B

FASTA31133,873
        10         20         30         40         50         60 
MSSEQAAAMG RVAVLMGGDS AEREISLISG RAVFEALRRR GVDAEAVDTA QVALAELTGF 

        70         80         90        100        110        120 
DRAFIALHGR GGEDGVIQGA LEALGVPYTG SGVLGCAIGM DKWRTKLLWR GAELPVPPGA 

       130        140        150        160        170        180 
LLRPETNRQA LIGSVGLPLM IKPAHEGSSI GMAKVERPEE LEAARAEAAR YDDLVLAERW 

       190        200        210        220        230        240 
IEGGEYTVAI LGEEALPAIR LETPRGFYDF EAKYRSGDTR YHCPAGLTEA EEQQIRQLAL 

       250        260        270        280        290        300 
DAFRVAGASG WGRVDFMRDR QGRFWLLEIN TIPGMTDHSL VPMAARAVGI DFDELCWRIL 

       310 
LDSRRREAGR P 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000453 Genomic DNA. Translation: ABI57532.1.
RefSeqYP_743022.1. NC_008340.1.

3D structure databases

ProteinModelPortalQ0A6K5.
SMRQ0A6K5. Positions 10-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187272.Mlg_2190.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI57532; ABI57532; Mlg_2190.
GeneID4270969.
KEGGaeh:Mlg_2190.
PATRIC20863637. VBIAlkEhr114327_2187.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycAEHR187272:GHAX-2237-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ALKEH
AccessionPrimary (citable) accession number: Q0A6K5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways