Reviewed, UniProtKB/Swiss-Prot Q0A6K3 (MURC_ALHEH)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Mlg_2192

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000336809
Regions
	Nucleotide binding	126 – 132	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0A6K3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 90902B34CC203F5B	FASTA	479	51,633
	10 20 30 40 50 60 MTDGDTSPAH TRPDAFGRVR HLHFVGIGGA GMGGIAEVLH NLGFTVTGSD LRENAITRRL 70 80 90 100 110 120 AGLGVQVVFG HEADHVQGAD AVVVSSAVQA DNPEVQAARE YRIPVVRRAE MLAELMRFRY 130 140 150 160 170 180 GIAVAGTHGK TTTTSLVASV LAEGGLDPTY VIGGRLNSSA SHARLGSGRY LVAEADESDA 190 200 210 220 230 240 SFLHLKPMMA VVTNIDADHL ETYGGDFDQL RHTFDEFLHH LPFYGLAVLC HDDPVLRDLG 250 260 270 280 290 300 PEIARQVRSY GFAEDADLRA VDIEQSGRRT RFTVVDGEAT FPVEVNLPGR HNVQNALAAI 310 320 330 340 350 360 AVARELHVDV AAIQRALQRF QGIGRRFQDH GTLRFGDARV TLVDDYGHHP REIAATLRAV 370 380 390 400 410 420 RDGWPGRRVL VVFQPHRYSR TRDLFEDFAR VLSEADALLV TEVYAAGEPP VAGATGRGLC 430 440 450 460 470 AAIRARGQVN PVFVETLEEL QQVLPGVARD GDLVLTLGAG SIGGAAAELA RRHGVEDGG

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI57534.1.
RefSeq	YP_743024.1.
3D structure databases
SMR	Q0A6K3. Positions 16-471.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A6K3.
Genome annotation databases
GeneID	4270971.
GenomeReviews	Gene locus Mlg_2192 in contig CP000453_GR.
KEGG	aeh:Mlg_2192.
NMPDR	fig|187272.6.peg.2089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	Q0A6K3.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ALHEH

Accession

Primary (citable) accession number: Q0A6K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)