Q0A5W1 (BIOB_ALHEH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	Mlg_2436

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola › Alkalilimnicola ehrlichii

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01694
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.
Sequence caution	The sequence ABI57776.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381191
Sites
	Metal binding	58	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	102	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	133	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	193	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	265	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A5W1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: 292C095BD0CEC0CC	FASTA	331	36,716
	10 20 30 40 50 60 MTATTQTPRH DWSKDEVLAL FEQPFNDLLH QAQTTHRAHF DPNTVQVSTL LSIKTGACPE 70 80 90 100 110 120 DCKYCPQSVR YDTGLEREQI LAVEEVVAAA RRARDAGATR FCMGAAWRSP KDRDLETVEA 130 140 150 160 170 180 MVREVKALGL ETCLTLGMLR DGQAERLREA GLDYYNHNLD TSEDYYDEII TTRSYQDRLD 190 200 210 220 230 240 TLARVRDAGL KTCCGGIIGM GETRQDRAEL LRTLASLPVQ PQSVPINQLV QVPGTPLHGV 250 260 270 280 290 300 EPPDPFEFVR TIAVARILMP ASYVRLSAGR EQMSDELQAL CFLAGANSIF YGDKLLTTGN 310 320 330 PEADKDRRLL ARLGMGFEAH ACAQAEAEDL G

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MLHE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI57776.1. Different initiation.
RefSeq	YP_743266.1. NC_008340.1.
3D structure databases
ProteinModelPortal	Q0A5W1.
ModBase	Search...
Protein-protein interaction databases
STRING	187272.Mlg_2436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI57776; ABI57776; Mlg_2436.
GeneID	4268742.
KEGG	aeh:Mlg_2436.
PATRIC	20864129. VBIAlkEhr114327_2429.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
ProtClustDB	CLSK2308029.
Enzyme and pathway databases
BioCyc	AEHR187272:GHAX-2488-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_ALHEH

Accession

Primary (citable) accession number: Q0A5W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	May 29, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program