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Q0A5W1 (BIOB_ALKEH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Mlg_2436
OrganismAlkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) [Complete proteome] [HAMAP]
Taxonomic identifier187272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeAlkalilimnicola

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ABI57776.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Biotin synthase HAMAP-Rule MF_01694
PRO_0000381191

Sites

Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1021Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1331Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1931Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2651Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0A5W1 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 292C095BD0CEC0CC

FASTA33136,716
        10         20         30         40         50         60 
MTATTQTPRH DWSKDEVLAL FEQPFNDLLH QAQTTHRAHF DPNTVQVSTL LSIKTGACPE 

        70         80         90        100        110        120 
DCKYCPQSVR YDTGLEREQI LAVEEVVAAA RRARDAGATR FCMGAAWRSP KDRDLETVEA 

       130        140        150        160        170        180 
MVREVKALGL ETCLTLGMLR DGQAERLREA GLDYYNHNLD TSEDYYDEII TTRSYQDRLD 

       190        200        210        220        230        240 
TLARVRDAGL KTCCGGIIGM GETRQDRAEL LRTLASLPVQ PQSVPINQLV QVPGTPLHGV 

       250        260        270        280        290        300 
EPPDPFEFVR TIAVARILMP ASYVRLSAGR EQMSDELQAL CFLAGANSIF YGDKLLTTGN 

       310        320        330 
PEADKDRRLL ARLGMGFEAH ACAQAEAEDL G 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000453 Genomic DNA. Translation: ABI57776.1. Different initiation.
RefSeqYP_743266.1. NC_008340.1.

3D structure databases

ProteinModelPortalQ0A5W1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187272.Mlg_2436.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI57776; ABI57776; Mlg_2436.
GeneID4268742.
KEGGaeh:Mlg_2436.
PATRIC20864129. VBIAlkEhr114327_2429.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OrthoDBEOG622PMP.
ProtClustDBCLSK2308029.

Enzyme and pathway databases

BioCycAEHR187272:GHAX-2488-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ALKEH
AccessionPrimary (citable) accession number: Q0A5W1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways