Q0A4T8 (HLDD_ALHEH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-L-glycero-D-manno-heptose-6-epimerase
EC=5.1.3.20

Alternative name(s):
ADP-L-glycero-beta-D-manno-heptose-6-epimerase
Short name=ADP-glyceromanno-heptose 6-epimerase
Short name=ADP-hep 6-epimerase
Short name=AGME

Gene names

Name:	hldD
Ordered Locus Names:	Mlg_2809

Organism

Alkalilimnicola ehrlichei (strain MLHE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

187272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Alkalilimnicola

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose By similarity. HAMAP MF_01601
Catalytic activity	ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose. HAMAP MF_01601
Cofactor	Binds 1 NADP+ per subunit By similarity. HAMAP MF_01601
Pathway	Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4. HAMAP MF_01601
Subunit structure	Homopentamer By similarity. HAMAP MF_01601
Domain	Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain By similarity. HAMAP MF_01601
Sequence similarities	Belongs to the sugar epimerase family. HldD subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	NADP
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW cellular metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ADP-glyceromanno-heptose 6-epimerase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	ADP-L-glycero-D-manno-heptose-6-epimerase HAMAP MF_01601		PRO_1000069341
Regions
	Nucleotide binding	10 – 11	2	NADP By similarity
	Nucleotide binding	31 – 32	2	NADP By similarity
	Nucleotide binding	75 – 79	5	NADP By similarity
	Region	200 – 203	4	Substrate binding By similarity
Sites
	Active site	139	1	Proton acceptor By similarity
	Active site	177	1	Proton acceptor By similarity
	Binding site	38	1	NADP By similarity
	Binding site	53	1	NADP By similarity
	Binding site	92	1	NADP By similarity
	Binding site	143	1	NADP By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	169	1	NADP; via amide nitrogen By similarity
	Binding site	177	1	NADP By similarity
	Binding site	179	1	Substrate; via carbonyl oxygen By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0A4T8 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A2564AAC3206B09E	FASTA	320	36,088
	10 20 30 40 50 60 MIIVTGGAGF IGSNLVHELN RRGRTDVIVV DNLTRGEKAL NLADCVIADY YDKDDFITLI 70 80 90 100 110 120 EADEGLGPVE AVFHLGACSA TTEWDGRYMM RNNFEYSRAL FHWCQDRRIP FIYASSAAVY 130 140 150 160 170 180 GGNSVFTEHP EHERPLNVYG YSKLAFDQYL RRYLDDLSAQ VVGLRYFNVY GPREQHKGGM 190 200 210 220 230 240 ASVVHHFSRQ LRESGQVRLF EGSDGYADGE QRRDFVDVSD CVRLKLWLLD HPEVSGIYNC 250 260 270 280 290 300 GTGRARTFNA MAHAVIDWFG HGEIEYIPFP EHLKGRYQSY TQADLSQLRA AGCDLAFNDI 310 320 ETGVRNYLDA VADAPAVMPS

References

[1]

"Complete sequence of Alkalilimnicola ehrilichei MLHE-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S., Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B., Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MLHE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000453 Genomic DNA. Translation: ABI58149.1.
RefSeq	YP_743639.1. NC_008340.1.
3D structure databases
ProteinModelPortal	Q0A4T8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0A4T8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4269152.
GenomeReviews	Gene locus Mlg_2809 in contig CP000453_GR.
KEGG	aeh:Mlg_2809.
NMPDR	fig|187272.6.peg.2683.
PATRIC	20864887. VBIAlkEhr114327_2804.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0451.
HOGENOM	HBG755066.
OMA	RRDFIYV.
PhylomeDB	Q0A4T8.
ProtClustDB	CLSK2780066.
Enzyme and pathway databases
BioCyc	AEHR187272:MLG_2809-MONOMER.
Family and domain databases
HAMAP	MF_01601. Heptose_epimerase. [Tree]
InterPro	IPR001509. Epimerase_deHydtase. IPR011912. Heptose_epim. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K03274.
PANTHER	PTHR10366:SF29. Heptose_epim. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02197. Heptose_epim. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HLDD_ALHEH

Accession

Primary (citable) accession number: Q0A4T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program